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product-image-AAA314245_AD10.png Application Data (Figure 3. Affinity ranking of different Rabbit anti-CD123 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Rabbit CD123 Monoclonal Antibody | anti-CD123 antibody

Anti-CD123 antibody (DM31), Rabbit mAb

Reactivity
Interleukin 3 receptor alpha
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Purified from cell culture supernatant by affinity chromatography
Synonyms
CD123, Antibody; Anti-CD123 antibody (DM31), Rabbit mAb; IL3R, IL3RA, IL-3Ra, IL-3R-alpha, IL3RAY, IL3RX, IL3RY, CD123 antigen, CD123, hIL3Ra, hIL-3Ra, MGC34174, IL-3 R alpha; anti-CD123 antibody
Ordering
Host
Rabbit
Reactivity
Interleukin 3 receptor alpha
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
DM31
Purity/Purification
Purified from cell culture supernatant by affinity chromatography
Form/Format
Preservative: 0.1% Procline 300 Constituents: 50% Glycerol; PBS, pH 7.4; 0.1% BSA
Applicable Applications for anti-CD123 antibody
FCM/FACS (Flow Cytometry), ELISA
Immunogen
Recombinant human CD123 (Thr19-Arg305)(PME100003) produced by using human HEK293 cells
Preparation and Storage
Store at -20 degree C for 12 months (Avoid repeated freezing and thawing)

Application Data

(Figure 3. Affinity ranking of different Rabbit anti-CD123 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

product-image-AAA314245_AD10.png Application Data (Figure 3. Affinity ranking of different Rabbit anti-CD123 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

FCM/FACS (Flow Cytometry)

(Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD123 monoclonal antibody (clone: DM31) on THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

product-image-AAA314245_FCM11.png FCM/FACS (Flow Cytometry) (Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD123 monoclonal antibody (clone: DM31) on THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Application Data

product-image-AAA314245_AD13.png Application Data

Application Data

(Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD123(right) were surface stained with Rabbit anti-CD123 monoclonal antibody 1μg/ml (clone: DM31) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)

product-image-AAA314245_AD15.png Application Data (Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD123(right) were surface stained with Rabbit anti-CD123 monoclonal antibody 1μg/ml (clone: DM31) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
Related Product Information for anti-CD123 antibody
Interleukin 3 receptor alpha (low affinity)(IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor alpha chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.
Product Categories/Family for anti-CD123 antibody

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Product Notes

The CD123 (Catalog #AAA314245) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD123 antibody (DM31), Rabbit mAb reacts with Interleukin 3 receptor alpha and may cross-react with other species as described in the data sheet. AAA Biotech's CD123 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the CD123 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD123, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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