<
WB (Western Blot)
(Detection of human CD127/IL-7R alpha by western blot. Samples: Whole cell lysate (25 ug) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD127 recombinant monoclonal antibody (AAA213628 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
CD127/IL-7R alpha Recombinant Antibody | CD127/IL-7Ralpha recombinant antibody
Rabbit anti-CD127/IL-7R alpha Recombinant Monoclonal Antibody [BLR177J]
Gene Names
IL7R; ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
CD127/IL-7R alpha, Recombinant Antibody; Rabbit anti-CD127/IL-7R alpha Recombinant Monoclonal Antibody [BLR177J]; IL7RA; IL-7R-alpha; interleukin-7 receptor subunit alpha; interleukin 7 receptor isoform H5-6; ILRA; IL-7RA; IL-7 receptor subunit alpha; CDW127; CD127 antigen; CD127; CD antigen CD127; IL-7R subunit alpha; interleukin 7 receptor alpha chain; Interleukin-7 receptor subunit alpha; interleukin 7 receptor; CD127/IL-7Ralpha recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR177J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for CD127/IL-7Ralpha recombinant antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 21-236 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD127/IL-7R alpha by western blot. Samples: Whole cell lysate (25 ug) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD127 recombinant monoclonal antibody (AAA213628 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human CD127/IL-7R alpha by western blot. Samples: Whole cell lysate (25 ug) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD127 recombinant monoclonal antibody (AAA213628 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human CD127/IL-7R alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from Daudi cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD127 recombinant monoclonal antibody (AAA213628 lot 1) used for IP at 6 ul per reaction. CD127 was also immunoprecipitated by a second antibody against a different epitope of CD127 (BL-1919A-4E2). For blotting immunoprecipitated CD127, AAA213628 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CD127/IL-7R alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from Daudi cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD127 recombinant monoclonal antibody (AAA213628 lot 1) used for IP at 6 ul per reaction. CD127 was also immunoprecipitated by a second antibody against a different epitope of CD127 (BL-1919A-4E2). For blotting immunoprecipitated CD127, AAA213628 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemisry)
(Detection of human CD127/IL-7R alpha in spleen by IHC. Antibody: Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody (AAA213628). Secondary: HRP-conjugated goat anti-rabbit IgG .Substrate: DAB.)
IHC (Immunohistochemisry)
(Detection of human CD127/IL-7R alpha in spleen by IHC. Antibody: Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody (AAA213628). Secondary: HRP-conjugated goat anti-rabbit IgG .Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human CD127/IL-7R alpha in metastatic lymph node by IHC. Antibody: Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody (AAA213628). Secondary: HRP-conjugated goat anti-rabbit IgG .Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human CD127/IL-7R alpha in metastatic lymph node by IHC. Antibody: Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody (AAA213628). Secondary: HRP-conjugated goat anti-rabbit IgG .Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human CD127/IL-7R alpha in Hodgkin lymphoma by IHC. Antibody: Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody (AAA213628). Secondary: HRP-conjugated goat anti-rabbit IgG .Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human CD127/IL-7R alpha in Hodgkin lymphoma by IHC. Antibody: Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody (AAA213628). Secondary: HRP-conjugated goat anti-rabbit IgG .Substrate: DAB.)
Related Product Information for CD127/IL-7Ralpha recombinant antibody
CD127/IL-7R alpha is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. IL7 is a hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. [taken from NCBI Entrez Gene (Gene ID: 3575)].
Product Categories/Family for CD127/IL-7Ralpha recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,581 Da
NCBI Official Full Name
interleukin-7 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 7 receptor
NCBI Official Symbol
IL7R
NCBI Official Synonym Symbols
ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
NCBI Protein Information
interleukin-7 receptor subunit alpha; IL-7RA; CD127 antigen; IL-7R subunit alpha; IL-7 receptor subunit alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6
UniProt Protein Name
Interleukin-7 receptor subunit alpha
UniProt Gene Name
IL7R
UniProt Synonym Gene Names
IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA
UniProt Entry Name
IL7RA_HUMAN
Similar Products
Product Notes
The CD127/IL-7Ralpha il7r (Catalog #AAA213628) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD127/IL-7R alpha Recombinant Monoclonal Antibody [BLR177J] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD127/IL-7R alpha can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CD127/IL-7Ralpha il7r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD127/IL-7R alpha, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.