WB (Western Blot)
(Western blot analysis of lysates from COLO205 cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The lane on the right is blocked with the phospho peptide.)
Rabbit anti-Human, Mouse IL-7R/CD127 Polyclonal Antibody | anti-IL-7R/CD127 antibody
IL-7R/CD127 (Phospho-Tyr449) Antibody
Gene Names
IL7R; ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
Reactivity
Human, Mouse
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
IL-7R/CD127, Antibody; IL-7R/CD127 (Phospho-Tyr449) Antibody; CD127 antigen; CDw127; IL-7R alpha; IL7RA; interleukin-7 receptor alpha chain; anti-IL-7R/CD127 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
IL-7R/CD127 (Phospho-Tyr449) Antibody detects endogenous levels of IL-7R/CD127 only when phosphorylated at Tyr449.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
252
Applicable Applications for anti-IL-7R/CD127 antibody
ELISA, IF (Immunofluorescence), WB (Western Blot)
Target Modification
Phospho
Modification Sites
H:Y449; M:Y449
Immunogen
The antiserum was produced against synthesized peptide derived from human IL-7R/CD127 around the phosphorylation site of Tyr449.
Immunogen Range
410-459
Preparation and Storage
Stable at -20 degree C for at least 1 year.
WB (Western Blot)
(Western blot analysis of lysates from COLO205 cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The lane on the right is blocked with the phospho peptide.)
WB (Western Blot)
(Western blot analysis of lysates from COLO205 cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The lane on the right is blocked with the phospho peptide.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HUVEC cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The picture on the right is blocked with the phospho peptide.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HUVEC cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The picture on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51 kDa
NCBI Official Full Name
interleukin-7 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 7 receptor
NCBI Official Symbol
IL7R
NCBI Official Synonym Symbols
ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
NCBI Protein Information
interleukin-7 receptor subunit alpha
UniProt Protein Name
Interleukin-7 receptor subunit alpha
UniProt Gene Name
IL7R
UniProt Synonym Gene Names
IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA
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Product Notes
The IL-7R/CD127 il7r (Catalog #AAA316405) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL-7R/CD127 (Phospho-Tyr449) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL-7R/CD127 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the IL-7R/CD127 il7r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-7R/CD127, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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