FCM/FACS (Flow Cytometry)
Mouse anti-Human CD152 Monoclonal Antibody | anti-CD152 antibody
anti-human CD152-R-PE
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD152, Antibody; anti-human CD152-R-PE; Monoclonal anti-human CD152 (CTLA-4)/R-PE; anti-CD152 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1κ
Clone Number
ANC152.2/8H5
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Applicable Applications for anti-CD152 antibody
FCM/FACS (Flow Cytometry)
Immunogen
Stimulated human PBL’s and human CD152 Ig fusion protein
Production
Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A565/A280 ratio of 3.81.
Performance
Anti-CD152/PE was tested for binding to CD152-muIg-coated 4u beads in FACS (Inquire). Five x 105 CD152-muIg coated beads per tube were spun down and incubated 45 minutes at 4°C with 80ul of anti-CD152/PE at a 1:50 dilution (10ug/ml). Tubes were washed twice, fixed and analyzed by FACS. Beads stained positive with a mean shift of 2.48 log10 fluorescent units when compared to a Mouse IgG1/PE Isotype control (Catalog #MBS666704). Binding was blocked when beads were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD152 antibody (Catalog #MBS666252).
Preparation and Storage
Store at 2 - 5°C. Freeze/Thawing is not recommended. Protect from light.
FCM/FACS (Flow Cytometry)
FCM/FACS (Flow Cytometry)
Related Product Information for anti-CD152 antibody
Human CD152 (CTLA-4) is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. Antibody ANC152.2 reacts with CTLA-4 expressed on activated T cells. ANC152.2 blocks binding of CD152 (CTLA-4) Ig fusion protein to its CD80/CD86 receptor.
References
T. Lindsten, et al, (1993) J Immunol 151: 3489-3499. T.L. Walunas, et al, (1994) Immunity 1:405-413. N.J. Karandikar, et al, (1996) J Exp Med 184: 783-788. A. Kitani, et al, (2000) J Immunol 165: 691-702.
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Product Notes
The CD152 (Catalog #AAA78174) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD152-R-PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD152 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD152 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD152, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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