Application Data
(Abatacept bridging ELISA for Anti-Drug Antibody (ADA) assay developmentA microtiter plate was coated overnight with abatacept at a concentration of 1 ug/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Abatacept Antibody, clone AbD37058ia (HCA335, black), clone AbD37065ia (HCA336, red) or Mouse Anti-Human CD152 . Detection was performed using HRP conjugated abatacept followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).)
Mouse anti-Human CD152 Monoclonal Antibody | anti-CD152 antibody
MOUSE ANTI HUMAN CD152
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, ELISA
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Synonyms
CD152, Antibody; MOUSE ANTI HUMAN CD152; CTLA-4; anti-CD152 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
BNI3
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Form/Format
Purified IgG - liquid
ALEXA FLUOR 647, FITC, Purified, RPE
Phosphate buffered saline
0.09% sodium azide (NaN3)
ALEXA FLUOR 647, FITC, Purified, RPE
Phosphate buffered saline
0.09% sodium azide (NaN3)
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-CD152 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA
Immunogen
Human CTLA-4/human IgG heavy chain fusion protein.
Preparation and Storage
IgG concentration 1.0mg/ml
Application Data
(Abatacept bridging ELISA for Anti-Drug Antibody (ADA) assay developmentA microtiter plate was coated overnight with abatacept at a concentration of 1 ug/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Abatacept Antibody, clone AbD37058ia (HCA335, black), clone AbD37065ia (HCA336, red) or Mouse Anti-Human CD152 . Detection was performed using HRP conjugated abatacept followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).)
Application Data
(Abatacept bridging ELISA for Anti-Drug Antibody (ADA) assay developmentA microtiter plate was coated overnight with abatacept at a concentration of 1 ug/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Abatacept Antibody, clone AbD37058ia (HCA335, black), clone AbD37065ia (HCA336, red) or Mouse Anti-Human CD152 . Detection was performed using HRP conjugated abatacept followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).)
Application Data
(Human Anti-Abatacept Antibody bridging ELISA for Pharmacokinetic (PK) assay developmentA microtiter plate was coated overnight with Mouse Anti-Human CD152 Antibody, clone BNI3 at a concentration of 1 ug/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of abatacept. Detection was performed using HRP conjugated Human Anti-Abatacept Antibody, clone AbD37058ia (HCA335) at a concentration of 2 ug/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).)
Application Data
(Human Anti-Abatacept Antibody bridging ELISA for Pharmacokinetic (PK) assay developmentA microtiter plate was coated overnight with Mouse Anti-Human CD152 Antibody, clone BNI3 at a concentration of 1 ug/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of abatacept. Detection was performed using HRP conjugated Human Anti-Abatacept Antibody, clone AbD37058ia (HCA335) at a concentration of 2 ug/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).)
Related Product Information for anti-CD152 antibody
Mouse anti Human CD152 antibody, clone BNI3 recognizes human CD152, also known as CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4), an inhibitory receptor and negative regulator of T-cell responses. CD152 is a single pass type 1 transmembrane protein belonging to the immunoglobulin superfamily containing a single Ig-v-like domain in the extracellular region.CD152 along with CD28 binds to the co-stimulatory molecules CD80 and CD86 (Azuma et al. 1993). Mouse anti human CD152 antibody, clone BNI3 is able to block ligand binding on the Raji B-cell line (Steiner et al. 2001) and blocks binding of an alternative clone, BNI8 to CTLA-4/Ig in ELISA. Mouse anti Human CD152 antibody, clone BNI3 binds to the same epitope as classified anti CTLA-4 clones 11D4 and 10A8 (Wang et al. In: Leukocyte typing VI 1997 Garland Publishing Inc. pp97-98, Bull World Health Organ. 1997). The cytoplasmic domain of CD152 contains a critical tyrosine at residue 201 phosphorylated by Janus Kinase 2 which subsequently controls surface expression through regulation of CD152 interaction with AP-2 (Shiratori et al. 1997, Chikuma et al. 2000). CD152 is expressed primarily as an intracellular antigen with transport to the cell surface under tight regulation of several molecules including Trim, PLD and TIRC7, CD152 also demonstrates rapid internalization once expressed at the cell surfac
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Product Notes
The CD152 (Catalog #AAA50478) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CD152 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD152 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the CD152 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD152, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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