Application Data
Mouse anti-Human CD16 Monoclonal Antibody | anti-CD16 antibody
anti-human CD16-Fab
Gene Names
FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD16, Antibody; anti-human CD16-Fab; Monoclonal anti-human CD16(FcgRIII) Fab; anti-CD16 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3G8
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions. No preservative added.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
233
Applicable Applications for anti-CD16 antibody
FCM/FACS (Flow Cytometry)
Performance
Five x 105 ficoll prepared human peripheral blood mononuclear cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD16 Fab diluted to 10 ug/ml. Cells were washed twice and incubated with 2° reagent Goat anti-Mouse IgG/FITC (Catalog #: ), after which they were washed three times, fixed and analyzed by FC. A net 6.8 % sub population of the cells stained positive with a mean shift of 1.21 log10 fluorescent units when compared to a Mouse IgG1 Fab Isotype control (Catalog # ).
Conjugation
Fab
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Immunoglobulin was enzymaticly cleaved using Immobilized Ficin. Fab monomer was separated from intact antibody, F(ab’)2 and Fc fragments by Protein A and Size exclusion chromatography. No intact antibody was observed by SDS-PAGE.
Immunogen
Human polymorphonuclear leukocytes
Preparation and Storage
Store at 2 - 5°C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for 12 months after shipping date when stored as recommended.
Product should retain activity for 12 months after shipping date when stored as recommended.
Application Data
Application Data
Related Product Information for anti-CD16 antibody
Information: Human CD16 is a low affinity receptor for complexed IgG and occurs in two distinct forms, as a glycosyl phosphtidyl inositol (GPI) anchored protein in granulocytes, and as an integral membrane protein on NK cells and macrophages. Antibody 3G8 recognizes both allelic forms of CD16. Antibody 3G8 blocks binding of complexed IgG to CD16.
References
H.B. Fleit, et al, (1982) Proc Natl Acad Sci USA 79: 3275-3279. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 574-597. G. Trinchieri & N. Valiante, (1993) Natural Immunity 12: 218-234. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 805-814. J.C. Edberg & R.P. Kimberly, (1997) J Immunol 159: 3849-3857.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
CD16, partial
NCBI Official Synonym Full Names
Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
NCBI Official Symbol
FCGR3A
NCBI Official Synonym Symbols
CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III-A; FcgammaRIIIA; CD16a antigen; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; igG Fc receptor III-2; Fc gamma receptor III-A; Fc-gamma receptor IIIb (CD16); neutrophil-specific antigen NA; Fc-gamma receptor III-2 (CD 16); immunoglobulin G Fc receptor III; Fc fragment of IgG, low affinity III, receptor for (CD16)
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-A
UniProt Gene Name
FCGR3A
UniProt Synonym Gene Names
CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa
UniProt Entry Name
FCG3A_HUMAN
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Product Notes
The CD16 fcgr3a (Catalog #AAA78150) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD16-Fab reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD16 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD16 fcgr3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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