Mouse CD16 Monoclonal Antibody | anti-CD16 antibody

MOUSE ANTI HUMAN CD16

Cat. Num. AAA11921

• Gene Names: FCGR3B; CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
• Applications: Flow Cytometry, Immunoprecipitation
• Synonyms: CD16, Antibody; MOUSE ANTI HUMAN CD16; CD16A, CD16B, FCG3, FCGR3, IGFR3, FcRIII; anti-CD16 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: LNK16
• Specificity: Mouse anti Human CD16 antibody, clone LNK16 recognizes CD16, a 50-65kDa cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. ; ; CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Mouse anti Human CD16 antibody, clone LNK16 has been reported to block the binding of IgG to the CD16 molecule (Tamm and Schmidt 1996).
• Form/Format: Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 233

• Applicable Applications for anti-CD16 antibody: Flow cytometry (FC), Immunoprecipitation (IP)
• Application Notes: FC - Suggested Dilution: 1/10 - 1/20; Use 10 ul of the suggested working dilution to label 10⁶ cells or 100ul human whole blood. ; ; Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
• Target Species: Human
• Preparation: Purified IgG prepared by ion exchange chromatography.
• Buffer Solution: Phosphate buffered saline
• Preservative Stabilisers: 0.09% Sodium Azide
• Immunogen: Normal human peripheral blood granulocytes.
• Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Application Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD16:Alexa Fluor 647 (AAA11921))

Application Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD16: FITC)

Application Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD16)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD16: Low Endotoxin)

Application Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD16: Biotin)

NCBI and Uniprot Product Information

• NCBI GI #: 132814489
• NCBI GeneID: 2215
• NCBI Accession #: NP_000561.3
• NCBI GenBank Nucleotide #: NM_000570.4
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor III-B isoform 2
• NCBI Official Synonym Full Names: Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
• NCBI Official Symbol: FCGR3B
• NCBI Official Synonym Symbols: CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma receptor IIIb (CD 16); fc-gamma RIII-beta; fc-gamma RIIIb; igG Fc receptor III-1
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III-B
• UniProt Gene Name: FCGR3B
• UniProt Synonym Gene Names: CD16B; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb
• UniProt Entry Name: FCG3B_HUMAN