IP (Immunoprecipitation)
(mFCGR3 was immunoprecipitated using:Lane A:0.5 mg RAW246.7 Whole Cell Lysate4uL anti-mFCGR3 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mFCGR3 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size :50 kDa)
Rabbit anti-Mouse CD16/FCGR3 Monoclonal Antibody | anti-FCGR3 antibody
Recombinant Anti-CD16/FCGR3 Antibody, Rabbit Monoclonal
Gene Names
Fcgr3; CD16
Reactivity
Mouse
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A
Synonyms
CD16/FCGR3, Antibody; Recombinant Anti-CD16/FCGR3 Antibody, Rabbit Monoclonal; CD16/FCGR3 Antibody, Rabbit MAb; Fc receptor, IgG, low affinity III; Anti-CD16 Antibody; anti-FCGR3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
015
Specificity
Mouse CD16/FCGR3.
No cross-reactivity in ELISA with Human CD32a, Human CD32b, Human CD16a, Human CD16b, Cynomolgus FCGR3
Has cross-reactivity in ELISA with Human CD64
No cross-reactivity in ELISA with Human CD32a, Human CD32b, Human CD16a, Human CD16b, Cynomolgus FCGR3
Has cross-reactivity in ELISA with Human CD64
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-FCGR3 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse CD16/FCGR3 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD16/FCGR3 (rM CD16/FCGR3).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(mFCGR3 was immunoprecipitated using:Lane A:0.5 mg RAW246.7 Whole Cell Lysate4uL anti-mFCGR3 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mFCGR3 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size :50 kDa)
IP (Immunoprecipitation)
(mFCGR3 was immunoprecipitated using:Lane A:0.5 mg RAW246.7 Whole Cell Lysate4uL anti-mFCGR3 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mFCGR3 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size :50 kDa)
WB (Western Blot)
(Anti-FCGR3 rabbit monoclonal antibody at 1:500 dilutionLane A: C6 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-FCGR3 rabbit monoclonal antibody at 1:500 dilutionLane A: C6 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-FCGR3 antibody
Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcgammaR). FcgammaR is divided into three classes, Fc gamma RI (CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcgammaRIIIa and CD16b/FcgammaRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
Product Categories/Family for anti-FCGR3 antibody
References
Edberg JC, et al. (2002) Genetic linkage and association of Fcgamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Low affinity immunoglobulin gamma Fc region receptor III
NCBI Official Synonym Full Names
Fc receptor, IgG, low affinity III
NCBI Official Symbol
Fcgr3
NCBI Official Synonym Symbols
CD16
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III
UniProt Gene Name
Fcgr3
UniProt Synonym Gene Names
IgG Fc receptor III; FcRIII
UniProt Entry Name
FCGR3_MOUSE
Similar Products
Product Notes
The FCGR3 fcgr3 (Catalog #AAA257137) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CD16/FCGR3 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD16/FCGR3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the FCGR3 fcgr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16/FCGR3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.