Application Data
(Staining of porcine peripheral blood mononuclear cells with Mouse anti Porcine CD163 : FITC)
Mouse CD163 Monoclonal Antibody | anti-CD163 antibody
MOUSE ANTI PIG CD163
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Synonyms
CD163, Antibody; MOUSE ANTI PIG CD163; M130; anti-CD163 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2A10/11
Specificity
Mouse anti Pig CD163 antibody, clone 2A10/11 recognises porcine CD163, a ~120 kDa single pass type 1 transmembrane cell surface glycoprotein expressed on cells of the monocyte/macrophage lineage.
Form/Format
Purified IgG - liquid
Phosphate buffered saline
Phosphate buffered saline
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
1110
Applicable Applications for anti-CD163 antibody
Immunohistochemistry Frozen, Immunohistochemistry-Paraffin , Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
Western Blot: Clone 2A10/11 detects a band of approximately 120kD in alveolar macrophage cell lysates under non-reducing conditions.
Flow Cytometry: 1/50-1/200
Western Blot: Clone 2A10/11 detects a band of approximately 120kD in alveolar macrophage cell lysates under non-reducing conditions.
Flow Cytometry: 1/50-1/200
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag.8.653 myeloma cell line.
Target Species
Pig
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Carrier free
Yes
Immunogen
Porcine alveolar macrophages.
Preparation and Storage
Store at 4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Data
(Staining of porcine peripheral blood mononuclear cells with Mouse anti Porcine CD163 : FITC)
Application Data
(Staining of porcine peripheral blood mononuclear cells with Mouse anti Porcine CD163 : FITC)
Application Data
(Staining of porcine peripheral blood mononuclear cells with Mouse anti Porcine CD163 (AAA12248))
Application Data
(Staining of porcine peripheral blood mononuclear cells with Mouse anti Porcine CD163 (AAA12248))
Related Product Information for anti-CD163 antibody
Mouse anti Pig CD163 antibody, clone 2A10/11 recognises porcine CD163, a 120kD single pass type 1 transmembrane cell surface glycoprotein expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. Expression of CD163 on peripheral blood monocytes varies between about 5% and 50% depending on the donor (Sanchez et al. 1999). Mouse anti Pig CD163, clone 2A10/11 is reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner (Sanchez-Torres et al. 2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
scavenger receptor cysteine-rich type 1 protein M130
NCBI Official Symbol
CD163
NCBI Protein Information
scavenger receptor cysteine-rich type 1 protein M130
UniProt Protein Name
Scavenger receptor cysteine-rich type 1 protein M130
UniProt Gene Name
CD163
UniProt Synonym Gene Names
M130; sCD163
UniProt Entry Name
C163A_PIG
Similar Products
Product Notes
The CD163 cd163 (Catalog #AAA12248) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD163 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry Frozen, Immunohistochemistry-Paraffin, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to 1x106 cells in 100ul. Western Blot: Clone 2A10/11 detects a band of approximately 120kD in alveolar macrophage cell lysates under non-reducing conditions. Flow Cytometry: 1/50-1/200. Researchers should empirically determine the suitability of the CD163 cd163 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD163, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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