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Application Data
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Mouse CD163 Monoclonal Antibody | anti-CD163 antibody

MOUSE ANTI RAT CD163

Gene Names
CD163; M130; MM130
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot
Synonyms
CD163, Antibody; MOUSE ANTI RAT CD163; anti-CD163 antibody
Ordering
 
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For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ED2
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5 mg/ml (varies by lot)
Sequence Length
1116
Applicable Applications for anti-CD163 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul
Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100
Immunohistology - Paraffin: Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Histology Positive Control Tissue
Liver
Buffer Solution
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Application Data

(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody , red an A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power)

Application Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)

Application Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)

Application Data

(Staining of rat peritoneal macrophages with Mouse anti Rat CD163: FITC)

Application Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)

Application Data

(Staining of rat peritoneal macrophages with Mouse anti Rat CD163: RPE)

Application Data

(Staining of acetone fixed, cryostat sectioned rat spleen with Mouse anti Rat CD163)

Application Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)

Application Data

(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody , red an A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power)

Related Product Information for anti-CD163 antibody
Mouse anti rat CD163, clone ED2 recognises the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007). Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990). Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
124,958 Da
NCBI Official Full Name
CD163
NCBI Official Synonym Full Names
CD163 molecule
NCBI Official Symbol
CD163
NCBI Official Synonym Symbols
M130; MM130
NCBI Protein Information
scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen
UniProt Protein Name
Scavenger receptor cysteine-rich type 1 protein M130
UniProt Gene Name
CD163
UniProt Synonym Gene Names
M130; sCD163
UniProt Entry Name
C163A_HUMAN

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Product Notes

The CD163 cd163 (Catalog #AAA12154) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD163 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/100 Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100 Immunohistology - Paraffin: Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Researchers should empirically determine the suitability of the CD163 cd163 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD163, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.