Application Data
(Published customer image:Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.Image caption:Specificity of IRF-7 polyclonal antibody. (A, B) 14M1.4 cells (A) and knockdown line KD#1 (B) were infected with L.donovani and stained at 24h for IRF-7. (C,D) L. donovani infected B6 mice (C) and B6.Irf7-/- mice (D) were infected for 5h and then stained for IRF-7.No phagosomal staining of IRF-7 was observed in KD#1 or in B6.Irf7-/- mice, confirming that this antibody does not cross react with Leishmania components.From: Phillips R, Svensson M, Aziz N, Maroof A, Brown N, et al. (2010) Innate Killing of Leishmania donovani by Macrophages of the Splenic Marginal Zone Requires IRF-7. PLoS Pathog 6(3): e1000813.)
Rat CD169 Monoclonal Antibody | anti-CD169 antibody
Rat Anti Mouse CD169: RPE
Application Data
(Published customer image:Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.Image caption:Specificity of IRF-7 polyclonal antibody. (A, B) 14M1.4 cells (A) and knockdown line KD#1 (B) were infected with L.donovani and stained at 24h for IRF-7. (C,D) L. donovani infected B6 mice (C) and B6.Irf7-/- mice (D) were infected for 5h and then stained for IRF-7.No phagosomal staining of IRF-7 was observed in KD#1 or in B6.Irf7-/- mice, confirming that this antibody does not cross react with Leishmania components.From: Phillips R, Svensson M, Aziz N, Maroof A, Brown N, et al. (2010) Innate Killing of Leishmania donovani by Macrophages of the Splenic Marginal Zone Requires IRF-7. PLoS Pathog 6(3): e1000813.)
Application Data
(Published customer image:Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.Image caption:Specificity of IRF-7 polyclonal antibody. (A, B) 14M1.4 cells (A) and knockdown line KD#1 (B) were infected with L.donovani and stained at 24h for IRF-7. (C,D) L. donovani infected B6 mice (C) and B6.Irf7-/- mice (D) were infected for 5h and then stained for IRF-7.No phagosomal staining of IRF-7 was observed in KD#1 or in B6.Irf7-/- mice, confirming that this antibody does not cross react with Leishmania components.From: Phillips R, Svensson M, Aziz N, Maroof A, Brown N, et al. (2010) Innate Killing of Leishmania donovani by Macrophages of the Splenic Marginal Zone Requires IRF-7. PLoS Pathog 6(3): e1000813.)
Application Data
(Published customer image:Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.Image caption:Irf-7-/- MZM and MMM are defective in anti-leishmanial activity.(A-F) Parasite localisation in wild type and Irf-7-/- mice. Spleens from naïve C57BL/6 (A) and Irf-7-/- (B) mice and C57BL/6 (C, E) and Irf-7-/- (D, F) mice infected with L.donovani for 5h or 24h, were stained for CD169 (green), SIGNR1 (red), Leishmania (white) and counterstained with DAPI (blue). Higher magnification inserts show localization of amastigotes (arrowed). (G) Parasite load in the MZ of C57BL/6 mice (open bars) and B6.Irf-7-/- mice (black bars) was determined by counting the number of parasites per MZ area *, p)
Application Data
(Published customer image:Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.Image caption:Irf-7-/- MZM and MMM are defective in anti-leishmanial activity.(A-F) Parasite localisation in wild type and Irf-7-/- mice. Spleens from naïve C57BL/6 (A) and Irf-7-/- (B) mice and C57BL/6 (C, E) and Irf-7-/- (D, F) mice infected with L.donovani for 5h or 24h, were stained for CD169 (green), SIGNR1 (red), Leishmania (white) and counterstained with DAPI (blue). Higher magnification inserts show localization of amastigotes (arrowed). (G) Parasite load in the MZ of C57BL/6 mice (open bars) and B6.Irf-7-/- mice (black bars) was determined by counting the number of parasites per MZ area *, p)
Application Data
(Published customer image:Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.Image caption:IRF-7 is induced in MZM and MM in situ. (A-D) Induction of IRF-7 in MMM and MZM. Naïve (A,B) and C57BL/6 mice infected for 24h (C, D) were stained for CD169 (A, C; green) or SIGNR1 (B, D; green), IRF-7 (red) and L. donovani (white). (E) The percentage of IRF-7+ parasite-containing phagosomes was quantified by counting 100 amastigotes/section (n = 3) and calculating the proportion of parasites with IRF-7 accumulation. *, p)
Application Data
(Published customer image:Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.Image caption:IRF-7 is induced in MZM and MM in situ. (A-D) Induction of IRF-7 in MMM and MZM. Naïve (A,B) and C57BL/6 mice infected for 24h (C, D) were stained for CD169 (A, C; green) or SIGNR1 (B, D; green), IRF-7 (red) and L. donovani (white). (E) The percentage of IRF-7+ parasite-containing phagosomes was quantified by counting 100 amastigotes/section (n = 3) and calculating the proportion of parasites with IRF-7 accumulation. *, p)
Application Data
(Published customer image:Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.Image caption:Splenic marginal zone macrophages and marginal metallophilic macrophages control L. donovani by an IFN-gamma independent mechanism. (A) L. donovani amastigotes are found predominantly in MMM and MZM after intravenous injection (purple, SIGNR1; green CD169; white, L. donovani; blue DAPI). (B) C57BL/6 mice were treated with 0.5mg XMG 1.6 anti-IFN-gamma IgG (black bars) or control IgG (open bars) and 2h later infected with L. donovani. At 5h and 24h p.i., the number of parasites per MZ area was determined. (C) 14M1.4 cells (black bars) and RAW 264.7 cells (open bars) were infected with L. donovani (MOI 10:1) and at indicated times p.i., parasite numbers per 100 macrophages were determined by fluorescence microscopy. *, p)
Application Data
(Published customer image:Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.Image caption:Splenic marginal zone macrophages and marginal metallophilic macrophages control L. donovani by an IFN-gamma independent mechanism. (A) L. donovani amastigotes are found predominantly in MMM and MZM after intravenous injection (purple, SIGNR1; green CD169; white, L. donovani; blue DAPI). (B) C57BL/6 mice were treated with 0.5mg XMG 1.6 anti-IFN-gamma IgG (black bars) or control IgG (open bars) and 2h later infected with L. donovani. At 5h and 24h p.i., the number of parasites per MZ area was determined. (C) 14M1.4 cells (black bars) and RAW 264.7 cells (open bars) were infected with L. donovani (MOI 10:1) and at indicated times p.i., parasite numbers per 100 macrophages were determined by fluorescence microscopy. *, p)
IF (Immunofluorescence)
(Immunoflourescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
IF (Immunofluorescence)
(Immunoflourescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
IF (Immunofluorescence)
(Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
IF (Immunofluorescence)
(Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
Application Data
(Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power)
Application Data
(Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power)
Application Data
(Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power)
Application Data
(Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power)
Application Data
(Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power)
Application Data
(Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power)
IHC (Immunohistochemistry)
(Immunohistochemical staining of frozen mouse spleen with Rat anti Mouse CD169 antibody, clone 3D6.112.)
IHC (Immunohistochemistry)
(Immunohistochemical staining of frozen mouse spleen with Rat anti Mouse CD169 antibody, clone 3D6.112.)
Application Data
(Figure A. RPE conjugated Rat anti Mouse CD45R and FITC conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD45R and FITC conjugated Rat anti Mouse CD169 . All experiments performed on murine bone marrow in the presence of murine SeroBlock (BUF041A).)
Application Data
(Figure A. RPE conjugated Rat anti Mouse CD45R and FITC conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD45R and FITC conjugated Rat anti Mouse CD169 . All experiments performed on murine bone marrow in the presence of murine SeroBlock (BUF041A).)
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Product Notes
The CD169 (Catalog #AAA12262) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD169 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD169 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD169, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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