IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithanti-CD169(AAA72427)SER-4(recombinantversion) ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsstainedwiththechimericrabbitIgGversionofSER-4(recombinantversion)(AAA72427)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72427,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
CD169 recombinant antibody
Anti-CD169 [SER-4 (recombinant version)], Rat IgG2a, kappa
Gene Names
Siglec1; Sn; Cd169; Siglec-1
Reactivity
Mouse
Applications
Immunohistochemistry
Purity
Protein A affinity purified
Synonyms
CD169, Recombinant Antibody; Anti-CD169 [SER-4 (recombinant version)], Rat IgG2a, kappa; CD169; Anti-CD169 [SER-4 (recombinant version)]; Sialoadhesin; Sheep erythrocyte receptor; SER; Sialic acid-binding Ig-like lectin 1; Siglec-1; CD169 recombinant antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a kappa
Clone Number
SER-4 (recombinant version)
Specificity
This antibody specifically recognize mouse CD169, which is a 180-185-kDa member of the Siglec family of macrophage-specific cell adhesion and endocytic receptors interacting with sialylated glycans. This receptor is expressed on a subset of macrophages that are found with high frequency in the spleen, liver, lymph node, and bone marrow. CD169 can also be detected on monocytes in the bone marrow and periphery.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for CD169 recombinant antibody
IHC (Immunohistochemistry)
Chimeric Use Statement
This constant region of this antibody has been modified to bind to Protein A, allowing highly-specific purification of this antibody by Protein A-affinity column.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithanti-CD169(AAA72427)SER-4(recombinantversion) ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsstainedwiththechimericrabbitIgGversionofSER-4(recombinantversion)(AAA72427)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72427,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithanti-CD169(AAA72427)SER-4(recombinantversion) ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsstainedwiththechimericrabbitIgGversionofSER-4(recombinantversion)(AAA72427)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72427,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
FCM/FACS (Flow Cytometry)
(FlowcytometryusingtheAnti-CD169antibodySER-4(recombinantversion)(AAA72427). ParaformaldehydefixedRAW264.7cellspermeabilizedwith0.5%Tritonwerestainedwithanti-unknownspecificityantibodyortherabbitIgGversionofSER-4(recombinantversion)(AAA72427,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(FlowcytometryusingtheAnti-CD169antibodySER-4(recombinantversion)(AAA72427). ParaformaldehydefixedRAW264.7cellspermeabilizedwith0.5%Tritonwerestainedwithanti-unknownspecificityantibodyortherabbitIgGversionofSER-4(recombinantversion)(AAA72427,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithanti-CD169(AAA72427)SER-4(recombinantversion) ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsstainedwiththechimericrabbitIgGversionofSER-4(recombinantversion)(AAA72427)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72427,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithanti-CD169(AAA72427)SER-4(recombinantversion) ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsstainedwiththechimericrabbitIgGversionofSER-4(recombinantversion)(AAA72427)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72427,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
Related Product Information for CD169 recombinant antibody
Spleen cells from AO rats immunised with thioglycollate-elicted murine peritoneal macrophages (TPM) that had been cutured in mouse serum to induce sheep erythrocyte receptor (SER).
References
Crocker PR, Gordon S. Mouse macrophage hemagglutinin (sheep erythrocyte receptor) with specificity for sialylated glycoconjugates characterized by a monoclonal antibody. J Exp Med. 1989 Apr 1;169(4):1333-46. N/A2926328
Note on publication:
Describes the making of SER-4 monoclonal antibody and characterizes the molecular nature and distribution on macrophage of SER with mAb SER-4 - inc data on IHC-Fr, IP and WB assays.
Note on publication:
Describes the making of SER-4 monoclonal antibody and characterizes the molecular nature and distribution on macrophage of SER with mAb SER-4 - inc data on IHC-Fr, IP and WB assays.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
172,769 Da
NCBI Official Full Name
Sialoadhesin
NCBI Official Synonym Full Names
sialic acid binding Ig-like lectin 1, sialoadhesin
NCBI Official Symbol
Siglec1
NCBI Official Synonym Symbols
Sn; Cd169; Siglec-1
NCBI Protein Information
sialoadhesin; SER; sheep erythrocyte receptor; sialic acid-binding Ig-like lectin 1
UniProt Protein Name
Sialoadhesin
UniProt Gene Name
Siglec1
UniProt Synonym Gene Names
Sa; Sn; SER; Siglec-1
UniProt Entry Name
SN_MOUSE
Similar Products
Product Notes
The CD169 siglec1 (Catalog #AAA72427) is a Recombinant Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD169 [SER-4 (recombinant version)], Rat IgG2a, kappa reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD169 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD169 siglec1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD169, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.