FCM/FACS (Flow Cytometry)
Mouse anti-Human CD17 Monoclonal Antibody | anti-CD17 antibody
anti-human CD17-FITC
Reactivity
Human
Synonyms
CD17, Antibody; anti-human CD17-FITC; Monoclonal anti-human CD17(lactosylceramide)/FITC; anti-CD17 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgMκ
Clone Number
Huly-m13/H018.3G-6.F5
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 500mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Performance
Five x 105 cultured Nalm-6 cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD17/FITC at a 1:50 dilution (5ug/ml). Cells were then washed three times, fixed and analyzed by FC. Cells stained positive with a mean shift of 1.38 log 10 fluorescent units when compared to a Mouse IgM/FITC negative control (Catalog # ). Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD17 antibody (Catalog # ).
Conjugation
FITC
Production
Antibody from (low FBS containing) tissue culture supernatant was size exclusion purified to 90% Immunoglobulin by SDS-PAGE, and reacted with FITC. Unconjugated FITC was removed from conjugate using a desalting column. Antibody conjugate is at 0.25 mg/ml with a Fluorescein/IgM molar ratio of 43.4
Immunogen
Human beta-2 microglobulin associated proteins from a detergent lysate of PBL
Preparation and Storage
Store at 2 - 5°C. Freeze/Thawing is not recommended. Protect from light.
FCM/FACS (Flow Cytometry)
FCM/FACS (Flow Cytometry)
Related Product Information for anti-CD17 antibody
Information: Antibodies to human CD17 react with lactosylceramide which is expressed on granulocytes, monocytes, platelets, and basophils (1). CD17 expression is down modulated on activated granulocytes. Antibody Huly-m13 recognizes CD17 on myeloid cells.
References
(1)Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 822-823.
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Product Notes
The CD17 (Catalog #AAA78166) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD17-FITC reacts with Human and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "CD17, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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