Mouse anti-Bovine CD172a Monoclonal Antibody | anti-SIRPA antibody
Mouse anti Bovine CD172a
Gene Names
SIRPA; MYD-1; PTPNS1
Reactivity
Bovine
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
Synonyms
CD172a, Antibody; Mouse anti Bovine CD172a; CD172a Antibody; anti-SIRPA antibody
Host
Mouse
Reactivity
Bovine
Clonality
Monoclonal
Isotype
IgG1
Clone Number
DH59B
Purity/Purification
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
Form/Format
Liquid
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
506
Applicable Applications for anti-SIRPA antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Immunogen
Cells from multiple species with the final screening of the fusion on cells from dog
Buffer Solution
Serum free tissue culture medium containing proprietary protein free supplement with 0.09% Sodium Azide (NaN3)
Species Cross Reactivity
Bovine, Bison, Water Buffalo, Sheep, Horse, Dog, Cat
N.B Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories peer-reviewed publications or personal communications from the originators.
N.B Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories peer-reviewed publications or personal communications from the originators.
Preparation and Storage
Store at +4 degree C. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.
Should this product contain a precipitate we recommend microcentrifugation before use.
Related Product Information for anti-SIRPA antibody
Mouse anti Bovine CD172a, clone DH59B, recognizes bovine CD172a also known as SIRP alpha or MyD-1 antigen. CD172a is a transmembrane signal regulatory protein expressed primarily by macrophages, monocytes, dendritic cells, granulocytes, myeloid progenitors, hematopoietic stem cells, and neurons (Barclay et al. 2006). The extracellular region of SIRP family consists of three immunoglobulin superfamily (IgSF) domains; two IgC and one IgV domain (Barclay et al. 2006).
The IgV domain of CD172a binds to CD47 (Hatherley et al. 2007).The binding domain of CD172a is analogous to that of immunoglobulins and T cell receptors and is involved in myeloid cell activation (Berg et al.2004). However, signaling via CD172a is mainly inhibitory to cell function and phagocytosis (Oldenborg et al. 2001). CD172a expressing cells are stimulated in Mycobacterium tuberculosis infection, influencing migration of dendritic cells and macrophages, phagocytosis, and granuloma formation (Waters et al. 2009).
The IgV domain of CD172a binds to CD47 (Hatherley et al. 2007).The binding domain of CD172a is analogous to that of immunoglobulins and T cell receptors and is involved in myeloid cell activation (Berg et al.2004). However, signaling via CD172a is mainly inhibitory to cell function and phagocytosis (Oldenborg et al. 2001). CD172a expressing cells are stimulated in Mycobacterium tuberculosis infection, influencing migration of dendritic cells and macrophages, phagocytosis, and granuloma formation (Waters et al. 2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
55,093 Da
NCBI Official Full Name
Tyrosine-protein phosphatase non-receptor type substrate 1
NCBI Official Symbol
SIRPA
NCBI Official Synonym Symbols
MYD-1; PTPNS1
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type substrate 1
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type substrate 1
UniProt Gene Name
SIRPA
UniProt Synonym Gene Names
MYD1; PTPNS1; SHPS1; SIRP; SHP substrate 1; SHPS-1; Sirp-alpha-1
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Product Notes
The SIRPA sirpa (Catalog #AAA50316) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Bovine CD172a reacts with Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's CD172a can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the SIRPA sirpa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD172a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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