Application Data
(Dog peripheral blood monocytes stained with Mouse anti Dog CD18 (AAA50190) followed by Rabbit F(ab')2 anti Mouse IgG:FITC)
Mouse CD18 Monoclonal Antibody | anti-CD18 antibody
MOUSE ANTI DOG CD18
Applications
Immunoprecipitation, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
CD18, Antibody; MOUSE ANTI DOG CD18; anti-CD18 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CA1.4E9
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
777
Applicable Applications for anti-CD18 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Dog
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Dog peripheral blood monocytes stained with Mouse anti Dog CD18 (AAA50190) followed by Rabbit F(ab')2 anti Mouse IgG:FITC)
Application Data
(Dog peripheral blood monocytes stained with Mouse anti Dog CD18 (AAA50190) followed by Rabbit F(ab')2 anti Mouse IgG:FITC)
Application Data
(Dog peripheral blood monocytes stained with Mouse anti Canine CD18: Alexa FluorR -647)
Application Data
(Dog peripheral blood monocytes stained with Mouse anti Canine CD18: Alexa FluorR -647)
Related Product Information for anti-CD18 antibody
Mouse anti Dog CD18 antibody, clone CA1.4E9 recognises the canine CD18 cell surface antigen, also known as the b2 integrin. The tissue and cellular distribution of CD18 in canine tissue closely follows that observed for humans (Moore et al. 1990) CD18 is expressed by virtually all leucocytes, but more strongly upon monocytes and granulocytes than on lymphocytes. The cross reactivity patterns of Mouse anti Canine CD18, clone CA1.4E9 further indicate that the antibody clone recognizes an epitope common to a number of mammalian species. Immunoprecipitation experiments using detergent lysatesof iodinated peripheral blood leukocytes indicate that clone Ca1.4E9 immunoprecipitates the common 95kDa beta 2 integrin chain (CD18) along with the non-covalently associated alpha chains at 180kDa (CD11a), 165kDa (CD11b) and 150kDa (CD11c) (Moore et al. 1990). Of note is the lack of precipitation of the integrin alpha D chain (CD11d), this is likely due to the almost complete absence of CD11d expression on peripheral blood lymphocytes, in contrast to expression in the splenic red pulp (Fry et al. 2003). High CD18 expression is a common feature of lymphoma and hyperplasia in dogs (Caniatti et al. 1996)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD18 protein
NCBI Official Symbol
cd18
NCBI Protein Information
CD18 protein
Similar Products
Product Notes
The CD18 (Catalog #AAA50190) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD18 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD18, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.