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Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse anti-Human CD19 Monoclonal Antibody | anti-CD19 antibody
CD19 (B-Lymphocyte Marker)
Gene Names
CD19; B4; CVID3
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Purity
Purified Ab WITHOUT BSA and Azide
Synonyms
CD19, Antibody; CD19 (B-Lymphocyte Marker); B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CVID3; Leu-12; T-cell surface antigen Leu-12; anti-CD19 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
CD19/3116
Specificity
CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages. In normal lymphoid tissue, CD19 is observed in germinal centers, in mantle zone cells, and in scattered cells of the inter-follicular areas. Anti-CD19 exhibits an overall immunoreactivity pattern similar to those of the antibodies against CD20 and CD22. However, in contrast to CD20, expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. Anti-CD19 positivity is seen in the vast majority of B-cell neoplasms commonly at a lower intensity than normal B-cell counterparts. Plasma cell neoplasms are nearly always negative, as are T-cell neoplasms.
Purity/Purification
Purified Ab WITHOUT BSA and Azide
Form/Format
Purified Ab WITHOUT BSA and Azide at 1.0mg/mL
Sequence Length
557
Applicable Applications for anti-CD19 antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA
Positive Control
Raji cells (FC). Tonsil, Lymph Node or Spleen (IHC).
Immunogen
Recombinant fragment of human CD19 protein (around aa96-281) (exact sequence is proprietary)
Cellular Localization
Cell membrane, Membrane raft
Research Areas
B Cell Markers, Cardiovascular, Complement System, Hematopoietic Stem Cells, Immune checkpoint, Immunology, Infectious Disease, Mesenchymal Stem Cell Differentiation, Signal Transduction
Preparation and Storage
Antibody without azide - store at -20 to -80 degrees C. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS_PAGE
(SDS-PAGE Analysis Purified CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116). Confirmation of Integrity and Purity of Antibody.)
SDS_PAGE
(SDS-PAGE Analysis Purified CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116). Confirmation of Integrity and Purity of Antibody.)
FCM/FACS (Flow Cytometry)
(Flow Cytometric Analysis of Raji cells using CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
FCM/FACS (Flow Cytometry)
(Flow Cytometric Analysis of Raji cells using CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116).)
References
Bregni, M., et al.1989. Blood 73: 753-762
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
95kDa
NCBI Official Full Name
B-lymphocyte antigen CD19 isoform 1
NCBI Official Synonym Full Names
CD19 molecule
NCBI Official Symbol
CD19
NCBI Official Synonym Symbols
B4; CVID3
NCBI Protein Information
B-lymphocyte antigen CD19
UniProt Protein Name
B-lymphocyte antigen CD19
UniProt Gene Name
CD19
UniProt Entry Name
CD19_HUMAN
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Product Notes
The CD19 cd19 (Catalog #AAA215292) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD19 (B-Lymphocyte Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD19 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the CD19 cd19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD19, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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