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product-image-AAA12268_APP7.jpg Application Data (Figure A. PE conjugated Mouse anti Human CD20 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. PE conjugated Mouse anti Human CD20 and Amethyst Orange conjugated Mouse anti Human CD19 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)

Mouse anti-Human CD19 Monoclonal Antibody | anti-CD19 antibody

Mouse Anti Human CD19: Amethyst Orange

Gene Names
CD19; B4; CVID3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Purified IgG from ascites prepared by precipitation and ion exchange chromatography.
Synonyms
CD19, Antibody; Mouse Anti Human CD19: Amethyst Orange; CD19 antibody, LT19; anti-CD19 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LT19
Purity/Purification
Purified IgG from ascites prepared by precipitation and ion exchange chromatography.
Form/Format
Purified IgG conjugated to Amethyst Orange - Liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
557
Applicable Applications for anti-CD19 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: Min. Dilution: Neat
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul
Preservative Stabilizers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin
Buffer Solution
Phosphate Buffered Saline
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use.

Application Data

(Figure A. PE conjugated Mouse anti Human CD20 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. PE conjugated Mouse anti Human CD20 and Amethyst Orange conjugated Mouse anti Human CD19 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)

product-image-AAA12268_APP7.jpg Application Data (Figure A. PE conjugated Mouse anti Human CD20 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. PE conjugated Mouse anti Human CD20 and Amethyst Orange conjugated Mouse anti Human CD19 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)

Application Data

(Figure A. PE conjugated Mouse anti Human CD22 and FITC conjugated Mouse IgG1 isotype control . Figure B. PE conjugated Mouse anti Human CD22 and FITC conjugated Mouse anti Human CD19 . All experiments performed on human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

product-image-AAA12268_APP6.jpg Application Data (Figure A. PE conjugated Mouse anti Human CD22 and FITC conjugated Mouse IgG1 isotype control . Figure B. PE conjugated Mouse anti Human CD22 and FITC conjugated Mouse anti Human CD19 . All experiments performed on human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data

(Figure A. Pacific Blue conjugated mouse anti human HLA ABC and A488 conjugated mouse IgG2a isotype control . Figure B. Pacific Blue conjugated mouse anti human HLA ABC and A488 conjugated mouse anti Human CD19 . All experiments performed on red cell lysed human blood gated on lymphocytes. Data acquired on the ZE5 cell analyzer)

product-image-AAA12268_APP5.jpg Application Data (Figure A. Pacific Blue conjugated mouse anti human HLA ABC and A488 conjugated mouse IgG2a isotype control . Figure B. Pacific Blue conjugated mouse anti human HLA ABC and A488 conjugated mouse anti Human CD19 . All experiments performed on red cell lysed human blood gated on lymphocytes. Data acquired on the ZE5 cell analyzer)

Application Data

(Figure A. FITC conjugated Rat anti Human CD18 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Rat anti Human CD18 and RPE conjugated Mouse anti Human CD19 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

product-image-AAA12268_APP4.jpg Application Data (Figure A. FITC conjugated Rat anti Human CD18 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Rat anti Human CD18 and RPE conjugated Mouse anti Human CD19 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

Application Data

(Figure A. Alexa488 conjugated mouse anti human CD4 and Pacific Blue conjugated Mouse IgG1 isotype control . Figure B. Alexa488 conjugated mouse anti human CD4 and Pacific Blue conjugated mouse anti human CD19 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)

product-image-AAA12268_APP3.jpg Application Data (Figure A. Alexa488 conjugated mouse anti human CD4 and Pacific Blue conjugated Mouse IgG1 isotype control . Figure B. Alexa488 conjugated mouse anti human CD4 and Pacific Blue conjugated mouse anti human CD19 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)

Application Data

(Figure A. RPE conjugated mouse anti human CD40 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti human CD40 and FITC conjugated mouse anti human CD19 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)

product-image-AAA12268_APP2.jpg Application Data (Figure A. RPE conjugated mouse anti human CD40 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti human CD40 and FITC conjugated mouse anti human CD19 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)

Application Data

(Figure A. FITC conjugated mouse anti human CD3 and Alexa 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD3 and Alexa 647 conjugated mouse anti human CD19 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock)

product-image-AAA12268_APP.jpg Application Data (Figure A. FITC conjugated mouse anti human CD3 and Alexa 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD3 and Alexa 647 conjugated mouse anti human CD19 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock)
Related Product Information for anti-CD19 antibody
Mouse anti Human CD19 antibody, clone LT19 recognizes human CD19 also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4. CD19 is a ~95 kDa type I single pass transmembrane glycoprotein expressed on follicular dendritic cells and B-cells during maturation but is lost on development into plasma cells (de Rie et al. 1989).
CD19 is the broadest lineage specific marker for B cells and functions as a B-cell co-receptor in conjunction with CD21 (Bradbury et al. 1992), CD9, CD81 and CD82 (Horváth et al. 1998). CD19 is implicated in the down-regulation of B cell growth and proliferation (Pezzutto et al. 1987).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
930
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
B-lymphocyte antigen CD19 isoform 1
NCBI Official Synonym Full Names
CD19 molecule
NCBI Official Symbol
CD19
NCBI Official Synonym Symbols
B4; CVID3
NCBI Protein Information
B-lymphocyte antigen CD19
UniProt Protein Name
B-lymphocyte antigen CD19
UniProt Gene Name
CD19
UniProt Entry Name
CD19_HUMAN

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Product Notes

The CD19 cd19 (Catalog #AAA12268) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD19: Amethyst Orange reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD19 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Min. Dilution: Neat Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul. Researchers should empirically determine the suitability of the CD19 cd19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD19, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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