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WB (Western Blot)
(Detection of mouse CD19 by western blot. Samples: Whole cell lysate (25 ug) from A20, J774A1, 70Z/3, NIH 3T3, and WEHI-231 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD19 recombinant monoclonal antibody (AAA213641 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
CD19 recombinant antibody
Rabbit anti-CD19 Recombinant Monoclonal Antibody [BLR208K]
Gene Names
Cd19; AW495831
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
CD19, Recombinant Antibody; Rabbit anti-CD19 Recombinant Monoclonal Antibody [BLR208K]; AW495831; B-lymphocyte antigen CD19; CD antigen CD19; differentiation antigen CD19; CD19 antigen; CD19 recombinant antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Clone Number
BLR208K
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for CD19 recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 497 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse CD19 by western blot. Samples: Whole cell lysate (25 ug) from A20, J774A1, 70Z/3, NIH 3T3, and WEHI-231 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD19 recombinant monoclonal antibody (AAA213641 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse CD19 by western blot. Samples: Whole cell lysate (25 ug) from A20, J774A1, 70Z/3, NIH 3T3, and WEHI-231 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD19 recombinant monoclonal antibody (AAA213641 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of mouse CD19 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from WEHI-231 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD19 recombinant monoclonal antibody (AAA213641 lot 1) used for IP at 6 ul/mg lysate. CD19 was also immunoprecipitated by a second antibody against a different epitope of CD19 (BL-2434-2B6). For blotting immunoprecipitated CD19, AAA213641 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of mouse CD19 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from WEHI-231 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD19 recombinant monoclonal antibody (AAA213641 lot 1) used for IP at 6 ul/mg lysate. CD19 was also immunoprecipitated by a second antibody against a different epitope of CD19 (BL-2434-2B6). For blotting immunoprecipitated CD19, AAA213641 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohiostchemistry)
(Detection of mouse CD19 by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-mouse CD19 recombinant monoclonal antibody (AAA213641 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohiostchemistry)
(Detection of mouse CD19 by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-mouse CD19 recombinant monoclonal antibody (AAA213641 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of mouse CD19 by immunocytochemistry. Sample: FFPE section of WEHI-231 cells. Antibody: Rabbit anti-mouse CD19 recombinant monoclonal antibody (AAA213641 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of mouse CD19 by immunocytochemistry. Sample: FFPE section of WEHI-231 cells. Antibody: Rabbit anti-mouse CD19 recombinant monoclonal antibody (AAA213641 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
Related Product Information for CD19 recombinant antibody
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulatio [taken from NCBI Entrez Gene (Gene ID: 930)].
Product Categories/Family for CD19 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,149 Da
NCBI Official Full Name
B-lymphocyte antigen CD19
NCBI Official Synonym Full Names
CD19 antigen
NCBI Official Symbol
Cd19
NCBI Official Synonym Symbols
AW495831
NCBI Protein Information
B-lymphocyte antigen CD19; differentiation antigen CD19
UniProt Protein Name
B-lymphocyte antigen CD19
UniProt Gene Name
Cd19
UniProt Entry Name
CD19_MOUSE
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Product Notes
The CD19 cd19 (Catalog #AAA213641) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD19 Recombinant Monoclonal Antibody [BLR208K] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD19 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CD19 cd19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD19, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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