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product-image-AAA11945_APP8.gif Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD19: RPE- Alexa Fluor 647)

Mouse CD19 Monoclonal Antibody | anti-CD19 antibody

MOUSE ANTI HUMAN CD19

Gene Names
CD19; B4; CVID3
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
CD19, Antibody; MOUSE ANTI HUMAN CD19; anti-CD19 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LT19
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
557
Applicable Applications for anti-CD19 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD19: RPE- Alexa Fluor 647)

product-image-AAA11945_APP8.gif Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD19: RPE- Alexa Fluor 647)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD19:FITC)

product-image-AAA11945_APP7.gif Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD19:FITC)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD19:Alexa Fluor 488)

product-image-AAA11945_APP6.gif Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD19:Alexa Fluor 488)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD19:RPE)

product-image-AAA11945_APP5.gif Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD19:RPE)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD19:Alexa Fluor 647)

product-image-AAA11945_APP4.gif Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD19:Alexa Fluor 647)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD19:Biotin)

product-image-AAA11945_APP3.gif Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD19:Biotin)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD19:Alexa Fluor 647)

product-image-AAA11945_APP2.gif Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD19:Alexa Fluor 647)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD19)

product-image-AAA11945_APP.gif Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD19)
Related Product Information for anti-CD19 antibody
Mouse anti Human CD19 antibody, clone LT19 recognizes human CD19 also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4. CD19 is a ~95kDa type I single pass transmembrane glycoprotein expressed on follicular dendritic cells and B-cells during maturation but is lost on development into plasma cells (de Rie et al. 1989). CD19 is the broadest lineage specific marker for B cells and functions as a B-cell co-receptor in conjunction with CD21(Bradbury et al. 1992), CD9, CD81 and CD82 (Horv¡th et al. 1998). CD19 is implicated in the down-regulation of B cell growth and proliferation (Pezzutto et al. 1987).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
930
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61,200 Da
NCBI Official Full Name
B-lymphocyte antigen CD19 isoform 1
NCBI Official Synonym Full Names
CD19 molecule
NCBI Official Symbol
CD19
NCBI Official Synonym Symbols
B4; CVID3
NCBI Protein Information
B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; T-cell surface antigen Leu-12; differentiation antigen CD19
UniProt Protein Name
B-lymphocyte antigen CD19
UniProt Gene Name
CD19
UniProt Entry Name
CD19_HUMAN

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Product Notes

The CD19 cd19 (Catalog #AAA11945) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD19 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the CD19 cd19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD19, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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