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Application Data
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Mouse CD20 Monoclonal Antibody | anti-CD20 antibody

MOUSE ANTI HUMAN CD20

Gene Names
MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Reactivity
Rhesus Monkey
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
CD20, Antibody; MOUSE ANTI HUMAN CD20; anti-CD20 antibody
Ordering
 
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For Research Use Only!
Host
Mouse
Reactivity
Rhesus Monkey
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2H7
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1mg/ml (varies by lot)
Sequence Length
130
Applicable Applications for anti-CD20 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD20: Pacific Blue (AAA11937PB))

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Alexa Fluor 488 (AAA11937A488))

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Biotin)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD20)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Alexa Fluor 647 (AAA11937A647))

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:FITC)

Related Product Information for anti-CD20 antibody
Mouse anti Human CD20 antibody, clone 2H7 recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein. The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells. The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyak et al. 2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
931
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,581 Da
NCBI Official Full Name
B-lymphocyte antigen CD20
NCBI Official Synonym Full Names
membrane-spanning 4-domains, subfamily A, member 1
NCBI Official Symbol
MS4A1
NCBI Official Synonym Symbols
B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
NCBI Protein Information
B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16
UniProt Protein Name
B-lymphocyte antigen CD20
UniProt Gene Name
MS4A1
UniProt Synonym Gene Names
CD20
UniProt Entry Name
CD20_HUMAN

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Product Notes

The CD20 ms4a1 (Catalog #AAA11937) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CD20 reacts with Rhesus Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD20 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the CD20 ms4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD20, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.