Mouse anti-Human CD205 Monoclonal Antibody | anti-CD205 antibody
Anti-Hu CD205 APC
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD205, Antibody; Anti-Hu CD205 APC; DEC-205; LY75; CLEC13B; lymphocyte antigen 75; anti-CD205 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
HD30
Specificity
The mouse monoclonal antibody HD30 recognizes an extracellular epitope of CD205, an approx. 200kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.
Purity/Purification
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Applicable Applications for anti-CD205 antibody
FCM/FACS (Flow Cytometry)
Conjugation
APC
Immunogen
Recombinant Fc-tagged human CD205
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Related Product Information for anti-CD205 antibody
CD205, also known as DEC-205, is an endocytic receptor of macrophage mannose receptor family. This 205kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells.
References
*Park CG, Rodriguez A, Ueta H, Lee H, Pack M, Matsuno K, Steinman RM: Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals. 2012 Mar 30;377(1-2):15-22., URL: http://www.ncbi.nlm.nih.gov/pubmed/22273672
*Mahnke K, Guo M, Lee S, Sepulveda H, Swain SL, Nussenzweig M, Steinman RM: The dendritic cell receptor for endocytosis, DEC-205, can recycle and enhance antigen presentation via major histocompatibility complex class II-positive lysosomal compartments. J Cell Biol. 2000 Oct 30;151(3):673-84., URL: http://www.ncbi.nlm.nih.gov/pubmed/11062267,,*Guo M, Gong S, Maric S, Misulovin Z, Pack M, Mahnke K, Nussenzweig MC, Steinman RM: A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum Immunol. 2000 Aug;61(8):729-38., URL: http://www.ncbi.nlm.nih.gov/pubmed/10980384,,*Gurer C, Strowig T, Brilot F, Pack M, Trumpfheller C, Arrey F, Park CG, Steinman RM, Münz C: Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses. Blood. 2008 Aug 15;112(4):1231-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18519810
*Mahnke K, Guo M, Lee S, Sepulveda H, Swain SL, Nussenzweig M, Steinman RM: The dendritic cell receptor for endocytosis, DEC-205, can recycle and enhance antigen presentation via major histocompatibility complex class II-positive lysosomal compartments. J Cell Biol. 2000 Oct 30;151(3):673-84., URL: http://www.ncbi.nlm.nih.gov/pubmed/11062267,,*Guo M, Gong S, Maric S, Misulovin Z, Pack M, Mahnke K, Nussenzweig MC, Steinman RM: A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum Immunol. 2000 Aug;61(8):729-38., URL: http://www.ncbi.nlm.nih.gov/pubmed/10980384,,*Gurer C, Strowig T, Brilot F, Pack M, Trumpfheller C, Arrey F, Park CG, Steinman RM, Münz C: Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses. Blood. 2008 Aug 15;112(4):1231-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18519810
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
198,311 Da
NCBI Official Full Name
LY75-CD302 fusion protein isoform 1
NCBI Official Synonym Full Names
LY75-CD302 readthrough
NCBI Official Symbol
LY75-CD302
NCBI Protein Information
LY75-CD302 fusion protein; DEC-205/DCL-1 fusion
UniProt Protein Name
Lymphocyte antigen 75
UniProt Gene Name
LY75
UniProt Synonym Gene Names
CD205; CLEC13B; Ly-75
UniProt Entry Name
LY75_HUMAN
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Product Notes
The CD205 ly75 (Catalog #AAA129131) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD205 APC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD205 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD205 ly75 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD205, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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