IF (Immunofluorescence)
(ImmunofluorescencestainingofDaudicellswithanti-CD23antibodyIDEC-152(AAA72533). ImmunofluorescenceanalysisofparaformaldehydefixedDaudicellsonShi-fixpluscoverslipsstainedwiththechimericrabbitIgGversionofIDEC-152(AAA72533)(1:100dilution)for1hourfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromlefttoright,toptobottom,AAA72533,DAPI,mergedchannels,andanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
CD23 recombinant antibody
Anti-CD23 [IDEC-152 (Lumiliximab, 6G5) ], Rabbit IgG, kappa
Gene Names
FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
Reactivity
Human
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
CD23, Recombinant Antibody; Anti-CD23 [IDEC-152 (Lumiliximab, 6G5) ], Rabbit IgG, kappa; CD23; Anti-CD23 [IDEC-152 (Lumiliximab, 6G5) ]; Gomiliximab; FCER2; BLAST-2; FcepsilonRII; Fc-epsilon-RII; Low affinity immunoglobulin epsilon Fc receptor; C-type lectin domain family 4 member J; Immunoglobulin E-binding factor; Lymphocyte IgE receptor; CD23 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
IDEC-152 (Lumiliximab, 6G5)
Specificity
This antibody is specific for human CD23.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for CD23 recombinant antibody
IP (Immunoprecipitation), FCM/FACS (Flow Cytometry)
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(ImmunofluorescencestainingofDaudicellswithanti-CD23antibodyIDEC-152(AAA72533). ImmunofluorescenceanalysisofparaformaldehydefixedDaudicellsonShi-fixpluscoverslipsstainedwiththechimericrabbitIgGversionofIDEC-152(AAA72533)(1:100dilution)for1hourfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromlefttoright,toptobottom,AAA72533,DAPI,mergedchannels,andanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofDaudicellswithanti-CD23antibodyIDEC-152(AAA72533). ImmunofluorescenceanalysisofparaformaldehydefixedDaudicellsonShi-fixpluscoverslipsstainedwiththechimericrabbitIgGversionofIDEC-152(AAA72533)(1:100dilution)for1hourfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromlefttoright,toptobottom,AAA72533,DAPI,mergedchannels,andanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
WB (Western Blot)
(Western Blotusinganti-CD23antibodyIDEC-152(Lumiliximab,6G5)(AAA72533). Daudicellslysate(35ugproteininRIPAbuffer)wasresolvedviaSDS-PAGE,andthesubsequentblotwasprobedwithAAA72533ataconcentrationof2ug/mlbeforedetectionusinganappropriatesecondaryantibody.Aprimaryincubationof1hourwasused,andproteinsweredetectedbychemiluminescence.)
WB (Western Blot)
(Western Blotusinganti-CD23antibodyIDEC-152(Lumiliximab,6G5)(AAA72533). Daudicellslysate(35ugproteininRIPAbuffer)wasresolvedviaSDS-PAGE,andthesubsequentblotwasprobedwithAAA72533ataconcentrationof2ug/mlbeforedetectionusinganappropriatesecondaryantibody.Aprimaryincubationof1hourwasused,andproteinsweredetectedbychemiluminescence.)
Related Product Information for CD23 recombinant antibody
The original chimeric antibody was generated from a monkey immunized with human CD23. Later on the chimeric version of the antibody was generated by cloning the variable regions of the antibody into human constant regions.
References
Rosenwasser et al. Allergic asthma and an anti-CD23 mAb (IDEC-152): results of a phase I, single-dose, dose-escalating clinical trial. J Allergy Clin Immunol. (2003); 112(3):563-70. N/A13679816
Note on publication:
Describes the potential use of this antibody in the treatment of allergic asthma.
Note on publication:
Describes the potential use of this antibody in the treatment of allergic asthma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
321
NCBI Official Full Name
Low affinity immunoglobulin epsilon Fc receptor
NCBI Official Synonym Full Names
Fc fragment of IgE, low affinity II, receptor for (CD23)
NCBI Official Symbol
FCER2
NCBI Official Synonym Symbols
CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
NCBI Protein Information
low affinity immunoglobulin epsilon Fc receptor; CD23 antigen; fc-epsilon-RII; lymphocyte IgE receptor; immunoglobulin E-binding factor; C-type lectin domain family 4, member J
UniProt Protein Name
Low affinity immunoglobulin epsilon Fc receptor
UniProt Gene Name
FCER2
UniProt Synonym Gene Names
CD23A; CLEC4J; FCE2; IGEBF
UniProt Entry Name
FCER2_HUMAN
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Product Notes
The CD23 fcer2 (Catalog #AAA72533) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD23 [IDEC-152 (Lumiliximab, 6G5) ], Rabbit IgG, kappa reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD23 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD23 fcer2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD23, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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