Mouse anti-Rat, Human CD235a Monoclonal Antibody | anti-CD235a antibody
Anti-Hu CD235a PE-Cy7
Gene Names
GYPA; MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
Reactivity
Rat, Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with tandem dye PE-Cy7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD235a, Antibody; Anti-Hu CD235a PE-Cy7; Glycophorin A; GYPA; GPA; PAS-2; Sialoglycoprotein alpha; MN sialoglycoprotein; GPSAT; GPErik; MSN blood group; glycophorin A (MNS blood group); anti-CD235a antibody
Host
Mouse
Reactivity
Rat, Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
JC159
Specificity
The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.
Purity/Purification
The purified antibody is conjugated with tandem dye PE-Cy7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Applicable Applications for anti-CD235a antibody
FCM/FACS (Flow Cytometry)
Conjugation
PE-Cy7
Immunogen
Membrane preparation from splenic hairy cell leukemia
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Related Product Information for anti-CD235a antibody
CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.
References
*Alijotas-Reig J, Palacio-Garcia C, Llurba E, Vilardell-Tarres M: Cell-derived microparticles and vascular pregnancy complications: a systematic and comprehensive review. Fertil Steril. 2013 Feb;99(2):441-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/23122952,*Maijenburg MW, Kleijer M, Vermeul K, Mul EP, van Alphen FP, van der Schoot CE, Voermans C: The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging. Haematologica. 2012 Feb;97(2):179-83., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=kleijer+m,*Beck Z, Jagodzinski LL, Eller MA, Thelian D, Matyas GR, Kunz AN, Alving CR: Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients. PLoS One. 2013 Nov 25;8(11):e81002., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=beck+z+ja,*Yamauchi T, Takenaka K, Urata S, Shima T, Kikushige Y, Tokuyama T, Iwamoto C, Nishihara M, Iwasaki H, Miyamoto T, Honma N, Nakao M, Matozaki T, Akashi K: Polymorphic Sirpa is the genetic determinant for NOD-based mouse lines to achieve efficient human cell engraftment. Blood. 2013 Feb 21;121(8):1316-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/23293079
*Nakahata T and Okumura N: Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers. Leuk Lymphoma. 1994;13: 401., URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgidb=p,*Yajima A, Urano-Tashiro Y, Shimazu K, Takashima E, Takahashi Y, Konishi K: Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane. Microbiol Immunol. 2008;52(2):69-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/18380804ordin,*Rogers CE, Bradley MS, Palsson BO et al: Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship withburst-forming units-erythroid. Exp Hematol. 1996; 24: 597., URL: https://www.ncbi.nlm.nih.gov/pubmed/8605964dopt=A,*Bain BJ: Leukemia Diagnosis: a guide to the FAB classification. Gower Medical Publishing; 1990., URL:,*Keren DF, Hanson CA and Hurtubise PE, eds.: Flow Cytometry and Clinical Diagnosis. Chicago, IL: ASCP Press; 1994., URL:
*Nakahata T and Okumura N: Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers. Leuk Lymphoma. 1994;13: 401., URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgidb=p,*Yajima A, Urano-Tashiro Y, Shimazu K, Takashima E, Takahashi Y, Konishi K: Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane. Microbiol Immunol. 2008;52(2):69-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/18380804ordin,*Rogers CE, Bradley MS, Palsson BO et al: Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship withburst-forming units-erythroid. Exp Hematol. 1996; 24: 597., URL: https://www.ncbi.nlm.nih.gov/pubmed/8605964dopt=A,*Bain BJ: Leukemia Diagnosis: a guide to the FAB classification. Gower Medical Publishing; 1990., URL:,*Keren DF, Hanson CA and Hurtubise PE, eds.: Flow Cytometry and Clinical Diagnosis. Chicago, IL: ASCP Press; 1994., URL:
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
13,000 Da
NCBI Official Full Name
Glycophorin-A
NCBI Official Synonym Full Names
glycophorin A (MNS blood group)
NCBI Official Symbol
GYPA
NCBI Official Synonym Symbols
MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
NCBI Protein Information
glycophorin-A
UniProt Protein Name
Glycophorin-A
UniProt Gene Name
GYPA
UniProt Synonym Gene Names
GPA
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Product Notes
The CD235a gypa (Catalog #AAA129173) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD235a PE-Cy7 reacts with Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD235a can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD235a gypa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD235a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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