IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithanti-CD24antibodyM1/69(AAA72556). Immunofluorescenceanalysisofparaformaldehyde-fixedRAW264.7cellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofM1/69(AAA72556)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72556,DAPI,mergedchannels,andanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
CD24 recombinant antibody
Anti-CD24 [M1/69], Rabbit IgG, kappa
Gene Names
Cd24a; HSA; Cd24; Ly-52; nectadrin
Reactivity
Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
CD24, Recombinant Antibody; Anti-CD24 [M1/69], Rabbit IgG, kappa; CD24; Anti-CD24 [M1/69]; Heat stable antigen; Signal transducer CD24; Lymphocyte antigen 52; Ly-52; M1/69-J11D heat stable antigen; HSA; Nectadrin; R13-Ag; X62 heat stable antigen; CD24 recombinant antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
M1/69
Specificity
This antibody binds mouse CD24 also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a variably glycosylated GPI-anchored membrane protein expressed on the surface lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. This protein plays an important role in differentiation of different cell types and its expression varies during lymphocyte differentiation which makes it a good marker for delineating various lymphocyte developmental stages. It is also expressed in erythroleukemia, small cell lung carcinoma and multiple sclerosis.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for CD24 recombinant antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), FCM/FACS (Flow Cytometry)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithanti-CD24antibodyM1/69(AAA72556). Immunofluorescenceanalysisofparaformaldehyde-fixedRAW264.7cellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofM1/69(AAA72556)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72556,DAPI,mergedchannels,andanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithanti-CD24antibodyM1/69(AAA72556). Immunofluorescenceanalysisofparaformaldehyde-fixedRAW264.7cellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofM1/69(AAA72556)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72556,DAPI,mergedchannels,andanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
WB (Western Blot)
(Western Blotusinganti-CD24antibodyM1/69(AAA72556). Mousespleentissuelysate(35ugproteininRIPAbuffer)wasresolvedviaSDS-PAGE,andthesubsequentblotwasprobedwiththechimericrabbitversionofM1/69(AAA72556)at0.0003ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hourwasused,andproteinsweredetectedbychemiluminescence.)
WB (Western Blot)
(Western Blotusinganti-CD24antibodyM1/69(AAA72556). Mousespleentissuelysate(35ugproteininRIPAbuffer)wasresolvedviaSDS-PAGE,andthesubsequentblotwasprobedwiththechimericrabbitversionofM1/69(AAA72556)at0.0003ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hourwasused,andproteinsweredetectedbychemiluminescence.)
Related Product Information for CD24 recombinant antibody
The original antibody was generated by immunizing DA rats with B10 mouse splenic T cells and concanavalin A-activated splenocytes.
References
Springer et al. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978 Aug;8(8):539-51. N/A81133
Note on publication:
Describes the generation and characterization of different antibodies against mouse cell surface antigens.
Note on publication:
Describes the generation and characterization of different antibodies against mouse cell surface antigens.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
7,797 Da
NCBI Official Full Name
heat stable antigen
NCBI Official Synonym Full Names
CD24a antigen
NCBI Official Symbol
Cd24a
NCBI Official Synonym Symbols
HSA; Cd24; Ly-52; nectadrin
NCBI Protein Information
signal transducer CD24
UniProt Protein Name
Signal transducer CD24
UniProt Gene Name
Cd24
UniProt Synonym Gene Names
Cd24a; Ly-52; Ly-52; HSA
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Product Notes
The CD24 cd24 (Catalog #AAA72556) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD24 [M1/69], Rabbit IgG, kappa reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD24 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD24 cd24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD24, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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