Mouse anti-Human CD274 Monoclonal Antibody | anti-CD274 antibody

anti-human CD274(B7-H1)-R-PE

Cat. Num. AAA78173

• Gene Names: CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay
• Synonyms: CD274, Antibody; anti-human CD274(B7-H1)-R-PE; Monoclonal anti-human CD274 (B7-H1, PD-L1)/R-PE; anti-CD274 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: ANC6H1
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative)
• Concentration: Volume: 0.2 ml (varies by lot)
• Sequence Length: 127

• Applicable Applications for anti-CD274 antibody: ELISA, FCM/FACS (Flow Cytometry)
• Performance: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 ug/ml PHA. Five x 105 cells per tube were washed and pre incubated 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-human CD274 at a dilution factor of 1:50 (20 ug/ml). They were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD274 antibody.
• Conjugation: PE
• Production: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 1 mg/ml with an A565/A280 ratio of 3.04.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Recombinant human CD274
• Preparation and Storage: Store at 2 - 5°C. Do not freeze! Protect from light.

Application Data

Related Product Information for anti-CD274 antibody

Information: CD274 (B7-H1, PD-L1, Programmed Death Ligand) is a member of the B7 family and is expressed on a variety of tissues including lymphoid cells. It plays an important role in regulation of T cell activation, and is involved in progression of cancer, arthritis and HIV infection (3). CD274 binding to its receptor CD279 (PD-1) on activated T cells can decrease proliferation. Conversely, ligation of CD279 on primed T cells can stimulate IL-10 production. High levels of CD274 present in Renal cell carcinoma is associated with poor prognosis (1). Tumor expressed CD274 can increase apoptosis of tumor specific T cells resulting in better tumor cell survival (2). Gamma Interferon and PHA can up regulate CD274 expression on T cells. Antibody ANC6H1 binds to recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC.

References

1)Cancer Research (April 2006) 66:3381. 2)J Molecular Medicine (Feb 2004) 81(5):281. 3)Int J Hematol (Nov 2003) 78(4):321.

NCBI and Uniprot Product Information

• NCBI GI #: 112363645
• NCBI GeneID: 29126
• NCBI Official Full Name: CD274, partial
• NCBI Official Synonym Full Names: CD274 molecule
• NCBI Official Symbol: CD274
• NCBI Official Synonym Symbols: B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• NCBI Protein Information: programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• UniProt Gene Name: CD274
• UniProt Synonym Gene Names: B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
• UniProt Entry Name: PD1L1_HUMAN