Application Data
(BALC/c mouse thymocytes were stained with Hamster Anti-Mouse CD28-UNLB followed by Goat Anti-Hamster IgG (H+L) Mouse/Rat ads-PE)
anti-Mouse CD28 Monoclonal Antibody | anti-Cd28 antibody
Hamster Anti-Mouse CD28-BIOT
Gene Names
Cd28; Cd28
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
CD28, Antibody; Hamster Anti-Mouse CD28-BIOT; Hamster Anti-Mouse CD28 - Biotin; Hamster Anti-Mouse CD28; anti-Cd28 antibody
Host
Syrian Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2
Clone Number
37.51
Specificity
Mouse CD28; Mr 40 kD
Form/Format
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3.
Sequence Length
63
Applicable Applications for anti-Cd28 antibody
FCM/FACS (Flow Cytometry)
Immunogen
C57BL/6N mouse T cell lymphoma EL-4 cell line
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
Preparation and Storage
Store at 2-8°C.
Application Data
(BALC/c mouse thymocytes were stained with Hamster Anti-Mouse CD28-UNLB followed by Goat Anti-Hamster IgG (H+L) Mouse/Rat ads-PE)
Application Data
(BALC/c mouse thymocytes were stained with Hamster Anti-Mouse CD28-UNLB followed by Goat Anti-Hamster IgG (H+L) Mouse/Rat ads-PE)
Related Product Information for anti-Cd28 antibody
CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) on B cells and other antigen presenting cells and plays an important role in the interaction between T cells and B cells. CD28 is a costimulatory receptor involved in many, but not all, T-cell independent immune responses
References
1. Gross, J. A., E. Callas, and J. P. Allison. 1992. J. Immunol. 149:380.
2. Giese, T., J. P. Allison, and W. F. Davidson. 1993. J. Immunol. 151:597.
3. Clements, J. L., G. Winslow, C. Donahue, S. M. Cooper, J. P. Allison, and R. C. Budd. 1993. Int. Immunol. 5:1451.
4. Nandi, D., J. A. Gross, and J. P. Allison. 1994. J. Immunol. 152:3361.
5. June, C.H., J.A. Bluestone, L.M. Nadler, and C.B. Thompson. 1992. Immunol. Today 15:321.
6. Gelfanov, V., Y. G. Lai, V. Gelfanova, J. Y. Dong, J. P. Su, and N. S. Liao. 1995. J. Immunol. 155:76.
7. Harding, F. A., J. G. McArthur, J. A. Gross, D. H. Raulet, and J. P. Allison. 1992. Nature 356:607.
8. Bluestone, J.A. 1995. Immunity 2:555.
2. Giese, T., J. P. Allison, and W. F. Davidson. 1993. J. Immunol. 151:597.
3. Clements, J. L., G. Winslow, C. Donahue, S. M. Cooper, J. P. Allison, and R. C. Budd. 1993. Int. Immunol. 5:1451.
4. Nandi, D., J. A. Gross, and J. P. Allison. 1994. J. Immunol. 152:3361.
5. June, C.H., J.A. Bluestone, L.M. Nadler, and C.B. Thompson. 1992. Immunol. Today 15:321.
6. Gelfanov, V., Y. G. Lai, V. Gelfanova, J. Y. Dong, J. P. Su, and N. S. Liao. 1995. J. Immunol. 155:76.
7. Harding, F. A., J. G. McArthur, J. A. Gross, D. H. Raulet, and J. P. Allison. 1992. Nature 356:607.
8. Bluestone, J.A. 1995. Immunity 2:555.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
CD28
NCBI Official Synonym Full Names
CD28 antigen
NCBI Official Symbol
Cd28
NCBI Official Synonym Symbols
Cd28
NCBI Protein Information
T-cell-specific surface glycoprotein CD28; OTTMUSP00000002157
UniProt Protein Name
T-cell-specific surface glycoprotein CD28
UniProt Entry Name
CD28_MOUSE
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Product Notes
The Cd28 (Catalog #AAA78671) is an Antibody produced from Syrian Hamster and is intended for research purposes only. The product is available for immediate purchase. The Hamster Anti-Mouse CD28-BIOT reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD28 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the Cd28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD28, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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