Mouse anti-Human CD28 Monoclonal Antibody | anti-CD28 antibody

CD28

Cat. Num. AAA215299

• Gene Names: CD28; Tp44
• Reactivity: Human
• Applications: Functional Assay, Flow Cytometry, Functional Assay
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD28, Antibody; CD28; T-cell-specific surface glycoprotein CD28; Tp44; anti-CD28 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: C28/76
• Specificity: Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 123

• Applicable Applications for anti-CD28 antibody: FA (Functional Assay), FCM/FACS (Flow Cytometry)
• Positive Control: Jurkat cells. Human lymph nodes and tonsils.
• Immunogen: Recombinant human CD28 protein
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 2q33.2
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified CD28 Mouse Monoclonal Antibody (C28/76). Confirmation of Integrity and Purity of Antibody.)

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD28 Mouse Monoclonal Antibody (C28/76) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).)

References

1. Jeong SH, Qiao M, Nascimbeni M, Hu Z, Rehermann B, Murthy K, Liang TJ. Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. J Virol. 2004;78(13):6995-7003. 2. Scharschmidt E, Wegener E, Heissmeyer V, Rao A, Krappmann D: Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol. 2004;24(9):3860-73.

NCBI and Uniprot Product Information

• NCBI GI #: 340545508
• NCBI GeneID: 940
• NCBI Accession #: NP_001230006.1
• NCBI GenBank Nucleotide #: NP_001230006.1
• UniProt Accession #: P10747
• Molecular Weight: 44kDa (monomer); 88kDa (dimer)
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 2
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_HUMAN