Hamster CD3 Monoclonal Antibody | anti-CD3 antibody

HAMSTER ANTI MOUSE CD3

Cat. Num. AAA49789

• Gene Names: Cd3e; CD3; T3e; AI504783; CD3epsilon
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry
• Synonyms: CD3, Antibody; HAMSTER ANTI MOUSE CD3; anti-CD3 antibody

• Host: Hamster
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 145-2C11
• Form/Format: Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-CD3 antibody: WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Fusion Partners: Spleen cells from hyperimmunized Armenian hamsters (Cricetulus migratorius) were fused with cells of the murine SP2/0 myeloma.
• Immunogen: H-2Kb - specific mouse cytotoxic T lymphocyte clone BM10-37.
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degrees C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degrees C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degrees C. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

IF (Immunofluorescence)

(Published customer image:Hamster anti Mouse CD3 antibody, clone 145-2C11 used for the identification of murine colonic lymphocytes by immunofluorescence.Image caption:Intestinal Myeloid Cells Respond Directly to Hh Signalling during Homeostasis and Inflammation (A–C) No DSS treatment. (D–F) 4 d of 3% DSS. (A, D) Few CD3+ T lymphocytes respond to Hh signalling during colonic homeostasis. Infiltrating T cells in DSS-induced ulcers do not express Gli1 or respond to Hh signalling. (B, E) Many but not all CD11b+ myeloid cells respond to Hh signalling and express LacZ. After 4 d of DSS, ulcers in Gli1+/lacZ animals are filled with myeloid cells, many of which respond to Hh signals. (C, F) CD11c+ dendritic cells respond directly to Hh signals during homeostasis and inflammation. Nuclear DAPI staining was performed but has been omitted to allow clear visualization of co-expression of nuclear LacZ and cell surface markers.From: Lees CW, Zacharias WJ, Tremelling M, Noble CL, Nimmo ER, Tenesa A, et al. (2008) Analysis of Germline GLI1 Variation Implicates Hedgehog Signalling in the Regulation of Intestinal Inflammatory Pathways.)

IF (Immunofluorescence)

(Published customer image:Hamster anti Mouse CD3 antibody, clone 145-2C11 used to identify T lymphocytes in mouse lamina propria by immunofluorescence.Image caption:Representative H&E staining of WT mice subjected to IRI or sham procedure with epithelial swelling and damage, cellular infiltrate, and signs of immune cell activation as well as apoptosis (left column). The immunohistological studies show constant numbers of CD3+ cells, a slightly decreased expression of F4/80 (tissue resident macrophages) and a strong increase of GR-1, which is expressed on neutrophils and activated macrophages. (GR-1, F4/80, or CD3 in red, DAPI positive nuclei in blue, autofluorescence of epithelial cells in green).From: Yu Y, Feng X, Vieten G, Dippel S, Imvised T, Gueler F, et al. (2017) Conventional alpha beta (αβ) T cells do not contribute to acute intestinal ischemia-reperfusion injury in mice.)

Application Data

(Figure A. RPE conjugated Rat anti Mouse CD22 and A488 conjugated Hamster IgG isotype control (Cat# please inquire).Figure B. RPE conjugated Rat anti Mouse CD22 and A488 conjugated Hamster anti Mouse CD3 (Cat # please inquire).All experiments were performed on mouse splenocytes in the presence of murine SeroBlock)

Related Product Information for anti-CD3 antibody

Hamster anti Mouse CD3 antibody, clone 145-2C11 detects CD3 epsilon (CD3e), a 20kDa transmembrane protein also known as CD3 or T3. CD3e is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell. CD3e is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. Hamster anti Mouse CD3 antibody, clone 145-2C11 is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.

NCBI and Uniprot Product Information

• NCBI GI #: 6680886
• NCBI GeneID: 12501
• NCBI Accession #: NP_031674.1
• NCBI GenBank Nucleotide #: NM_007648.4
• UniProt Accession #: P22646
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3 antigen, epsilon polypeptide
• NCBI Official Symbol: Cd3e
• NCBI Official Synonym Symbols: CD3; T3e; AI504783; CD3epsilon
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: Cd3e
• UniProt Entry Name: CD3E_MOUSE