Mouse anti-Human CD3 Monoclonal Antibody | anti-CD3E antibody

CD3 (T-Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA62812

• Gene Names: CD3E; T3E; TCRE; IMD18
• Reactivity: Human
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: CD3, Antibody; CD3 (T-Cell Marker) Mouse Monoclonal Antibody; CD3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex; anti-CD3E antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: B-B12
• Specificity: Reacts with five invariable CD3 chains (designated as and ) with molecular weight ranging from 16-28kDa. CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.
• Form/Format: 200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
• Sequence Length: 207

• Applicable Applications for anti-CD3E antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Cell Surface and Cytoplasmic
• Immunogen: Human T cell leukemia cells
• Human Chromosome Location: 11q23.3
• Positive Control: Jurkat cells. Tonsil and lymph node
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(Purified CD3e Mouse Monoclonal Antibody (B-B12). Confirmation of Integrity and Purity of Antibody.)

IF (Immunofluorescence)

(Immunofluorescence Analysis of lurkat cells labeling CD3e with CD3e Mouse Monoclonal Antibody (B-B12) followed by Goat anti-mouse I G-CF488 (Green). The nuclear counterstain is Reddot (Red).)

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of Jurkat cells. CD3e Mouse Monoclonal Antibody (B-BI2) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red))

References

Kishimoto T. et al., eds. Leukocyte Typing VI, p44-48 and p1111, Garland Publishing, Inc, New York and London, 1997.Meuer SC et al. Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor. Nature 1983, 303(5920):808-810 Reinherz et al., Cell 30, 715, (1982) Borst et al., J. Biol. Chem. 258, 5135, (1983) Van den Elsen et al., Nature 312, (1984) Furley et al., Cell 46, 75, (1986) Gold et al., Nature 321, 4, (1986) Oettgen and Terhorst, Hum. Immunol. 18, 187, (1987) Clevers et al., Ann. Rev. Immunol. 6, 629, (1988

NCBI and Uniprot Product Information

• NCBI GI #: 4502671
• NCBI GeneID: 916
• NCBI Accession #: NP_000724.1
• NCBI GenBank Nucleotide #: NM_000733.3
• UniProt Accession #: P07766; A8K997
• Molecular Weight: 20kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN