Rat CD3 Monoclonal Antibody | anti-CD3 antibody

RAT ANTI HUMAN CD3:FITC

Cat. Num. AAA49585

• Gene Names: CD3E; T3E; TCRE; IMD18
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD3, Antibody; RAT ANTI HUMAN CD3:FITC; anti-CD3 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: CD3-12
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 207

• Applicable Applications for anti-CD3 antibody: FCM/FACS (Flow Cytometry)
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Immunogen: Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val- Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC )
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Species Cross Reactivity: Reacts with: Bovine, Dog, Horse, Rhesus Monkey, Pig, Chicken, Mouse, Duck, Koala, Harbour Porpoise, Alpaca, Cynomolgus monkey, Spotted Hyena, Sea Lion, Cat, Amazon Parrot, Raccoon, Great horned owl (Bubo virginianus), Bullfrog, Xenopus Based on sequence similarity, is expected to react with:Mammals, Birds, Amphibia N.B. Antibody reactivity and working conditions may vary between species.
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CD3 antibody

Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, gamma, delta, ζ) that assemble and function as three pairs of dimers (ε gamma, εdelta, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005)

NCBI and Uniprot Product Information

• NCBI GI #: 4502671
• NCBI GeneID: 916
• NCBI Accession #: NP_000724.1
• NCBI GenBank Nucleotide #: NM_000733.3
• UniProt Accession #: P07766; A8K997
• Molecular Weight: 23,147 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule, epsilon (CD3-TCR complex)
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN