IHC (Immunohiostchemistry)
(Immunohistochemistry of CD3 epsilon in paraffin-embedded Human tonsil using CD3 epsilon Rabbit mAb at dilution 1/50)
anti-Human CD3 epsilon Monoclonal Antibody | anti-CD3epsilon antibody
CD3 epsilon Rabbit mAb
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
CD3 epsilon, Antibody; CD3 epsilon Rabbit mAb; T3E; TCRE; IMD18; anti-CD3epsilon antibody
Host
Recombinant Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
S02-1B5
Purity/Purification
Affinity Purified
Form/Format
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration
0.3mg/ml (varies by lot)
Applicable Applications for anti-CD3epsilon antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide of human CD3 epsilon
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycles.
IHC (Immunohiostchemistry)
(Immunohistochemistry of CD3 epsilon in paraffin-embedded Human tonsil using CD3 epsilon Rabbit mAb at dilution 1/50)
IHC (Immunohiostchemistry)
(Immunohistochemistry of CD3 epsilon in paraffin-embedded Human tonsil using CD3 epsilon Rabbit mAb at dilution 1/50)
WB (Western Blot)
(Western blot detection of CD3 epsilon in Jurkat cell lysates using CD3 epsilon Rabbit mAb(1:500 diluted).Predicted band size:23kDa.Observed band size:23kDa.)
WB (Western Blot)
(Western blot detection of CD3 epsilon in Jurkat cell lysates using CD3 epsilon Rabbit mAb(1:500 diluted).Predicted band size:23kDa.Observed band size:23kDa.)
Related Product Information for anti-CD3epsilon antibody
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).
Product Categories/Family for anti-CD3epsilon antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
207
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
NCBI Official Synonym Full Names
CD3e molecule, epsilon (CD3-TCR complex)
NCBI Official Symbol
CD3E
NCBI Official Synonym Symbols
T3E; TCRE; IMD18
NCBI Protein Information
T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Gene Name
CD3E
UniProt Synonym Gene Names
T3E
UniProt Entry Name
CD3E_HUMAN
Similar Products
Product Notes
The CD3epsilon cd3e (Catalog #AAA314326) is an Antibody produced from Recombinant Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD3 epsilon Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 epsilon can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD3epsilon cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3 epsilon, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.