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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with CD3 epsilon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Rabbit anti-Human CD3 epsilon Monoclonal Antibody | anti-CD3e antibody
CD3 epsilon Antibody
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
CD3 epsilon, Antibody; CD3 epsilon Antibody; CD3 epsilon antibody; CD3e antibody; CD3e antigen epsilon polypeptide (TiT3 complex) antibody; CD3E antigen epsilon polypeptide antibody; CD3E antigen; epsilon subunit antibody; CD3e molecule epsilon antibody; CD3e molecule; epsilon (CD3 TCR complex) antibody; epsilon (CD3-TCR complex) antibody; CD3E_HUMAN antibody; IMD18 antibody; T cell antigen receptor complex epsilon subunit of T3 antibody; T cell surface antigen T3/Leu 4 epsilon chain antibody; T cell surface glycoprotein CD3 epsilon chain antibody; T-cell surface antigen T3/Leu-4 epsilon chain antibody; T-cell surface glycoprotein CD3 epsilon chain antibody; T3E antibody; TCRE antibody; anti-CD3e antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
SY0239
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CD3e antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with CD3 epsilon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with CD3 epsilon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of CD3 epsilon on different lysates using anti-CD3 epsilon antibody at 1/1, 000 dilution. Positive control: Lane 1: Jurkat Lane 2: Human thymus)
WB (Western Blot)
(Western blot analysis of CD3 epsilon on different lysates using anti-CD3 epsilon antibody at 1/1, 000 dilution. Positive control: Lane 1: Jurkat Lane 2: Human thymus)
Related Product Information for anti-CD3e antibody
The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). CD3 is composed of four polypeptides: zeta, gamma, epsilon and delta . Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase. TCR ligation also induces a conformational change in CD3epsilon, such that a proline region is exposed and then associates with the adaptor protein Nck.
Product Categories/Family for anti-CD3e antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
207
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
NCBI Official Synonym Full Names
CD3e molecule, epsilon (CD3-TCR complex)
NCBI Official Symbol
CD3E
NCBI Official Synonym Symbols
T3E; TCRE; IMD18
NCBI Protein Information
T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Gene Name
CD3E
UniProt Synonym Gene Names
T3E
UniProt Entry Name
CD3E_HUMAN
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Product Notes
The CD3e cd3e (Catalog #AAA312193) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD3 epsilon Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 epsilon can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD3e cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3 epsilon, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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