Application Data
Mouse anti-Human CD30 Monoclonal Antibody | anti-CD30 antibody
anti-human CD30-R-PE
Reactivity
Human
Applications
Flow Cytometry
Synonyms
CD30, Antibody; anti-human CD30-R-PE; Monoclonal anti-human CD30(Ki-1)/R-PE; anti-CD30 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2bκ
Clone Number
AC10
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as preservative).
Applicable Applications for anti-CD30 antibody
Flow Cytometry (FC)
Application Notes
Working Dilution: 1:50 (or use 1.6 ul of concentrated stock per 5 x 105-cell test)
Performance
Five x 105 cultured CEM human tumor cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD30/R-PE at a 1:50 dilution (10ug/ml). Cells were washed three times, fixed and analyzed by FC. Cells stained positive with a mean shift of 0.51 log10 fluorescent units when compared to a Mouse IgG2b/PE negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD30 antibody
Production
Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography.Antibody conjugate is at 0.75 mg/ml with a PE : mAb molar ratio of 0.61.
QC Test Method
FC Binding to CEM cells:
Specification: >0.4 log shift ; Result: 0.82 log shift.
Specification: >0.4 log shift ; Result: 0.82 log shift.
Preparation and Storage
Store at 2 - 5°C. Do not freeze! Protect from light.
Application Data
Application Data
Related Product Information for anti-CD30 antibody
Information: Human CD30 is a member of the tumor necrosis factor (TNF) receptor family and is expressed on mitogen activated B and T cells. Antibody AC10 recognizes the CD30 molecule of about 120 kd.
References
M.A. Bowen, et al, (1993) J Immunol 151: 5896-5906. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1115-1122. B. Falini, et al, (1995) Blood 85: 1-14.
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Product Notes
The CD30 (Catalog #AAA14859) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD30-R-PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD30 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC). Working Dilution: 1:50 (or use 1.6 ul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD30 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD30, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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