IHC (Immunohistochemistry)
(Human Hodgkin's lymphoma tissue was stained with Anti-rabbit anti CD30 antibody (Cat# AAA71423) at 1:50 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Rabbit anti-Human CD30 Polyclonal Antibody | anti-CD30 antibody
Rabbit anti CD30
Gene Names
TNFRSF8; CD30; Ki-1; D1S166E
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The rabbit polyclonal antibody is purified by affinity purification.
Synonyms
CD30, Antibody; Rabbit anti CD30; Rabbit anti-CD30; anti-CD30 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
This antibody reacts with human. The other species are not tested.
Purity/Purification
The rabbit polyclonal antibody is purified by affinity purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Applicable Applications for anti-CD30 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Antigen Preparation
Recombinant protein encoding aa 1-150 of human CD30 protein.
Positive Control
Raji cells. Hodgkin's lymphoma
Cellular Location
Cell Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
IHC (Immunohistochemistry)
(Human Hodgkin's lymphoma tissue was stained with Anti-rabbit anti CD30 antibody (Cat# AAA71423) at 1:50 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
IHC (Immunohistochemistry)
(Human Hodgkin's lymphoma tissue was stained with Anti-rabbit anti CD30 antibody (Cat# AAA71423) at 1:50 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Related Product Information for anti-CD30 antibody
CD30, a single chain glycoprotein, is synthesized as a 90kDa precursor which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. The CD30/Ki-1 antigen is expressed by mononuclear Hodgkin and multinucleated Reed-Sternberg cells in Hodgkin? disease, by the tumor cells of a majority of anaplastic large cell lymphomas, and by a varying proportion of activated T and B cells.
References
Newman, P.J., et al. Science 247, 1219, (1990)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
120kDa/95kDa
NCBI Official Full Name
CD30 antigen
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 8
NCBI Official Symbol
TNFRSF8
NCBI Official Synonym Symbols
CD30; Ki-1; D1S166E
NCBI Protein Information
tumor necrosis factor receptor superfamily member 8; CD30 antigen; Ki-1 antigen; CD30L receptor; OTTHUMP00000001783; OTTHUMP00000221481; cytokine receptor CD30; lymphocyte activation antigen CD30
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 8
UniProt Gene Name
TNFRSF8
UniProt Synonym Gene Names
CD30; D1S166E
UniProt Entry Name
TNR8_HUMAN
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Product Notes
The CD30 tnfrsf8 (Catalog #AAA71423) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti CD30 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD30 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD30 tnfrsf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD30, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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