Mouse anti-Human CD31 Monoclonal Antibody | anti-CD31 antibody
CD31 Monoclonal Antibody
IHC: 1:50-1:500
IF: 1:50-1:200
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Application Data
(Overlay histogram showing HL-60 cells stained with AAA27009 (red line) at 1:500. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C.The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.)
Application Data
(Overlay histogram showing THP-1 cells stained with AAA27009 (red line) at 1:500. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C.The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.)
IF (Immunofluorescence)
(Immunofluorescence staining of THP-1 cells with AAA27009 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells with AAA27009 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IHC (Immunohistchemistry)
(IHC image of AAA27009 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC image of AAA27009 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC image of AAA27009 diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC image of AAA27009 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC image of AAA27009 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Western BlotPositive WB detected in: Jurkat whole cell lysate, THP-1 whole cell lysate, Raji whole cell lysateAll lanes: CD31 antibody at 2.5ug/mlSecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 83, 81, 80, 82 KDaObserved band size: 130 KDa)
