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product-image-AAA23931_APP8.jpg Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using CD31 Mouse Monoclonal Antibody (PECAM1/3527) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Mouse anti-Human CD31/PECAM-1 Monoclonal Antibody | anti-PECAM1 antibody

CD31/PECAM-1 (Endothelial Cell Marker)

Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
CD31/PECAM-1, Antibody; CD31/PECAM-1 (Endothelial Cell Marker); EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'; anti-PECAM1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
PECAM1/3527
Specificity
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
738
Applicable Applications for anti-PECAM1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Application Notes
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Positive Control
Jurkat cells. Tonsil or Angiosarcoma.
Immunogen
Recombinant fragment (around aa 625-738) of human CD31 protein (exact sequence is proprietary)
Cellular Localization
Cell Surface and cytoplasm of endothelial cells
Hu-chromosome Location
17q23.3
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD31 Mouse Monoclonal Antibody (PECAM1/3527) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

product-image-AAA23931_APP8.jpg Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using CD31 Mouse Monoclonal Antibody (PECAM1/3527) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified CD31 Mouse Monoclonal Antibody (PECAM1/3527). Confirmation of Integrity and Purity of Antibody.)

product-image-AAA23931_SDS_PAGE7.jpg SDS-PAGE (SDS-PAGE Analysis Purified CD31 Mouse Monoclonal Antibody (PECAM1/3527). Confirmation of Integrity and Purity of Antibody.)

IF (Immunofluorescence)

(Immunofluorescence staining of Jurkat cells using CD31 Mouse Monoclonal Antibody (PECAM1/3527) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot)

product-image-AAA23931_IF6.jpg IF (Immunofluorescence) (Immunofluorescence staining of Jurkat cells using CD31 Mouse Monoclonal Antibody (PECAM1/3527) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot)

FCM (Flow Cytometry)

(Flow Cytometric Analysis of Jurkat cells using CD31 Mouse Monoclonal Antibody (PECAM1/3527) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

product-image-AAA23931_FCM5.jpg FCM (Flow Cytometry) (Flow Cytometric Analysis of Jurkat cells using CD31 Mouse Monoclonal Antibody (PECAM1/3527) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human tonsil stained with Purified CD31 Mouse Monoclonal Antibody (PECAM1/3527).)

product-image-AAA23931_IHC4.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human tonsil stained with Purified CD31 Mouse Monoclonal Antibody (PECAM1/3527).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human prostate stained with CD31 Mouse Monoclonal Antibody (PECAM1/3527).)

product-image-AAA23931_IHC3.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human prostate stained with CD31 Mouse Monoclonal Antibody (PECAM1/3527).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human prostate stained with CD31 Mouse Monoclonal Antibody (PECAM1/3527).)

product-image-AAA23931_IHC2.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human prostate stained with CD31 Mouse Monoclonal Antibody (PECAM1/3527).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human prostate stained with CD31 Mouse Monoclonal Antibody (PECAM1/3527).)

product-image-AAA23931_IHC.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human prostate stained with CD31 Mouse Monoclonal Antibody (PECAM1/3527).)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~100kDa (endothelium) and ~130kDa (platelets)
NCBI Official Full Name
platelet endothelial cell adhesion molecule
NCBI Official Synonym Full Names
platelet and endothelial cell adhesion molecule 1
NCBI Official Symbol
PECAM1
NCBI Official Synonym Symbols
CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
NCBI Protein Information
platelet endothelial cell adhesion molecule
UniProt Protein Name
Platelet endothelial cell adhesion molecule
UniProt Gene Name
PECAM1
UniProt Synonym Gene Names
PECAM-1
UniProt Entry Name
PECA1_HUMAN

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Product Notes

The PECAM1 pecam1 (Catalog #AAA23931) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD31/PECAM-1 (Endothelial Cell Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD31/PECAM-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. FC/FACS: 1-2ug/million cells IF: 1-2ug/ml Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the PECAM1 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD31/PECAM-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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