FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human CD31 antibody. THP-1 cells were stained with an irrelevant antibody (Green Histogram) or an anti-human CD31 antibody monoclonal antibody (#AAA120766, Blue Histogram) at a concentration of 5ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.)
Mouse anti-Human CD31/PECAM1 Monoclonal Antibody | anti-CD31/PECAM1 antibody
Anti-Human CD31/PECAM1 Antibody (SAA2179)
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human
Applications
Western Blot, ELISA
Purity
>95% as determined by SDS-PAGE.Protein A/G purified from cell culture supernatant.
Synonyms
CD31/PECAM1, Antibody; Anti-Human CD31/PECAM1 Antibody (SAA2179); Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD31; PECAM1; VHH-mFc; anti-CD31/PECAM1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
SAA2179
Purity/Purification
>95% as determined by SDS-PAGE.
Protein A/G purified from cell culture supernatant.
Protein A/G purified from cell culture supernatant.
Form/Format
Liquid; 0.01M PBS, pH 7.4.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD31/PECAM1 antibody
WB (Western Blot), ELISA
Preparation and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4 degree C short term (1-2 weeks). Store at -20 degree C 12 months. Store at -80 degree C long term.
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human CD31 antibody. THP-1 cells were stained with an irrelevant antibody (Green Histogram) or an anti-human CD31 antibody monoclonal antibody (#AAA120766, Blue Histogram) at a concentration of 5ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human CD31 antibody. THP-1 cells were stained with an irrelevant antibody (Green Histogram) or an anti-human CD31 antibody monoclonal antibody (#AAA120766, Blue Histogram) at a concentration of 5ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human CD31 antibody. Jurkat cells were stained with an irrelevant antibody (Green Histogram) or an anti-human CD31 antibody monoclonal antibody (#AAA120766, Blue Histogram) at a concentration of 5ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human CD31 antibody. Jurkat cells were stained with an irrelevant antibody (Green Histogram) or an anti-human CD31 antibody monoclonal antibody (#AAA120766, Blue Histogram) at a concentration of 5ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.)
WB (Western Blot)
(Various lysates were subjected to SDS PAGE followed by western blot with CD31 antibody (AAA120766) at 1 ug/mL.Lane 1: THP-1 cell lysateLane 2: Jurkat cell lysateSecond Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 ug/mL.Predict MW: 83 kDa)
WB (Western Blot)
(Various lysates were subjected to SDS PAGE followed by western blot with CD31 antibody (AAA120766) at 1 ug/mL.Lane 1: THP-1 cell lysateLane 2: Jurkat cell lysateSecond Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 ug/mL.Predict MW: 83 kDa)
Product Categories/Family for anti-CD31/PECAM1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,536 Da
NCBI Official Full Name
platelet endothelial cell adhesion molecule
NCBI Official Synonym Full Names
platelet/endothelial cell adhesion molecule 1
NCBI Official Symbol
PECAM1
NCBI Official Synonym Symbols
CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
NCBI Protein Information
platelet endothelial cell adhesion molecule; CD31 antigen
UniProt Protein Name
Platelet endothelial cell adhesion molecule
UniProt Gene Name
PECAM1
UniProt Synonym Gene Names
PECAM-1
UniProt Entry Name
PECA1_HUMAN
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Product Notes
The CD31/PECAM1 pecam1 (Catalog #AAA120766) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human CD31/PECAM1 Antibody (SAA2179) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD31/PECAM1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CD31/PECAM1 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD31/PECAM1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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