Application Data
(Surface Staining of HT-29 / SP2 cells with Mouse anti Human CD324:RPE)
Mouse anti-Human CD324 Monoclonal Antibody | anti-CD324 antibody
Mouse Anti Human CD324
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
>95% by SDS PAGEPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Synonyms
CD324, Antibody; Mouse Anti Human CD324; CD324 antibody, 67A4; anti-CD324 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
67A4
Purity/Purification
>95% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Form/Format
Purified IgG - Liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
882
Applicable Applications for anti-CD324 antibody
WB (Western Blot), IP (Immunoprecipitation), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Immunogen
T-47D cells
Preservative Stabilizers
<0.1% Sodium Azide (NaN3)
Buffer Solution
Phosphate Buffered Saline
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE.
This product should be stored undiluted.
This product should be stored undiluted.
Application Data
(Surface Staining of HT-29 / SP2 cells with Mouse anti Human CD324:RPE)
Application Data
(Surface Staining of HT-29 / SP2 cells with Mouse anti Human CD324:RPE)
Application Data
(Surface Staining of HT-29 cells with Mouse anti Human CD324:FITC)
Application Data
(Surface Staining of HT-29 cells with Mouse anti Human CD324:FITC)
Application Data
(Surface Staining of HT-29 / SP2 cells with Mouse anti Human CD324:APC)
Application Data
(Surface Staining of HT-29 / SP2 cells with Mouse anti Human CD324:APC)
Related Product Information for anti-CD324 antibody
Mouse anti Human CD324, clone 67A4 recognizes CD324 also known as E-Cadherin, a transmembrane glycoprotein which mediates calcium dependant cell adhesion and cell junction formation.
The extracellular domain mediates a Ca2+ dependant homophillic interaction with a cadherin molecule on the surface of a neighboring cell. This leads to the formation of tight junctions which assist in blocking the movement of cells and facilitating cell-cell interactions (Gloushankova et al. 2017).
The cytoplasmic domain is able to interact with catenins which link the CD324 to the actin cytoskeleton which help mediate downstream signaling events growth inhibitory signals to prevent uncontrolled tissue growth during embryonic development, tissue regeneration, wound healing. Loss of CD324 expression has been associated with tumor progression and metastasis. It is thought that metastasis is more likely to occur due to the loss of a maintained stable physical link between tumor cells (Mendonsa et al. 2018).
This clone 67A4 has been used in immunocytochemistry experiments to examine the localization of CD324 on medullary epithelial cells (Kutleša et al. 2002).
The extracellular domain mediates a Ca2+ dependant homophillic interaction with a cadherin molecule on the surface of a neighboring cell. This leads to the formation of tight junctions which assist in blocking the movement of cells and facilitating cell-cell interactions (Gloushankova et al. 2017).
The cytoplasmic domain is able to interact with catenins which link the CD324 to the actin cytoskeleton which help mediate downstream signaling events growth inhibitory signals to prevent uncontrolled tissue growth during embryonic development, tissue regeneration, wound healing. Loss of CD324 expression has been associated with tumor progression and metastasis. It is thought that metastasis is more likely to occur due to the loss of a maintained stable physical link between tumor cells (Mendonsa et al. 2018).
This clone 67A4 has been used in immunocytochemistry experiments to examine the localization of CD324 on medullary epithelial cells (Kutleša et al. 2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cadherin-1 isoform 1 preproprotein
NCBI Official Synonym Full Names
cadherin 1
NCBI Official Symbol
CDH1
NCBI Official Synonym Symbols
UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
NCBI Protein Information
cadherin-1
UniProt Protein Name
Cadherin-1
UniProt Gene Name
CDH1
UniProt Synonym Gene Names
CDHE; UVO; E-cadherin
UniProt Entry Name
CADH1_HUMAN
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Product Notes
The CD324 cdh1 (Catalog #AAA50387) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD324 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD324 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD324 cdh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD324, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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