IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD34 Monoclonal Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Mouse anti-Human CD34 Monoclonal Antibody | anti-CD34 antibody
CD34 Antibody (Center)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
CD34, Antibody; CD34 Antibody (Center); Hematopoietic progenitor cell antigen CD34; CD34; anti-CD34 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1κ
Clone Number
132CT29.1.5
Specificity
This CD34 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 185-214 amino acids from the Central region of human CD34.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
185-214
Sequence Length
385
Applicable Applications for anti-CD34 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD34 Monoclonal Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD34 Monoclonal Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysate from HUVEC cell line, using CD34 Antibody (Center). AAA283447 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from HUVEC cell line, using CD34 Antibody (Center). AAA283447 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Related Product Information for anti-CD34 antibody
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.
Product Categories/Family for anti-CD34 antibody
References
Genome-wide association analysis identifies multiple loci related to resting heart rate. Eijgelsheim M, et al. Hum Mol Genet, 2010 Oct 1. PMID 20639392. The effect of CXCL12 processing on CD34+ cell migration in myeloproliferative neoplasms. Cho SY, et al. Cancer Res, 2010 Apr 15. PMID 20388788. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614. Junctional adhesion molecule A expressed on human CD34+ cells promotes adhesion on vascular wall and differentiation into endothelial progenitor cells. Stellos K, et al. Arterioscler Thromb Vasc Biol, 2010 Jun. PMID 20378847. Human gallbladder carcinoma: Role of neurotrophins, MIB-1, CD34 and CA15-3. Artico M, et al. Eur J Histochem, 2010 Mar 11. PMID 20353905.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40716
NCBI Official Full Name
hematopoietic progenitor cell antigen CD34 isoform a
NCBI Official Synonym Full Names
CD34 molecule
NCBI Official Symbol
CD34
NCBI Protein Information
hematopoietic progenitor cell antigen CD34
UniProt Protein Name
Hematopoietic progenitor cell antigen CD34
UniProt Gene Name
CD34
UniProt Entry Name
CD34_HUMAN
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Product Notes
The CD34 cd34 (Catalog #AAA283447) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 185-214. The CD34 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD34 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the CD34 cd34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD34, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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