Mouse anti-Human CD3E Monoclonal Antibody | anti-CD3E antibody

Mouse anti-CD3E Monoclonal Antibody [UCHT1]

Cat. Num. AAA213512

• Gene Names: CD3E; T3E; TCRE; IMD18
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified
• Synonyms: CD3E, Antibody; Mouse anti-CD3E Monoclonal Antibody [UCHT1]; CD3-epsilon; TCRE; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; CD antigen CD3e; IMD18; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T3E; CD3 epsilon subunit of T-cell receptor complex; anti-CD3E antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: UCHT1
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-CD3E antibody: FCM/FACS (Flow Cytometry), WB (Western Blot)
• Immunogen: Human infant thymocytes and Sezary cells
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human CD3E by western blot. Samples: Whole cell lysate (50 ug) from MOLT-4, HEK293T, CCRF-CEM, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Mouse anti-CD3E monoclonal antibody [UCHT1] (AAA213512 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

FCM/FACS (Flow Cytometry)

(Detection of human CD3E (shaded) in Jurkat cells by flow cytometry. Antibody: Mouse anti-CD3 recombinant monoclonal antibody [UCHT1] (AAA213512) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG .)

Related Product Information for anti-CD3E antibody

CD3E is the CD3-epsilon polypeptide, which together with CD3-gamma,-delta and-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The epsilon polypeptide plays an essential role in T-cell development [taken from NCBI Entrez Gene (Gene ID: 916)].

Product Categories/Family for anti-CD3E antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4502671
• NCBI GeneID: 916
• NCBI Accession #: NP_000724.1
• NCBI GenBank Nucleotide #: NM_000733.3
• UniProt Accession #: P07766; A8K997
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule, epsilon (CD3-TCR complex)
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN