Application Data
(Human peripheral blood platelets stained with Mouse anti Human CD42a:FITC (AAA50233))
Mouse CD42a Monoclonal Antibody | anti-CD42a antibody
MOUSE ANTI HUMAN CD42a:FITC
Gene Names
GP9; GPIX; CD42a
Applications
Flow Cytometry, Functional Assay
Synonyms
CD42a, Antibody; MOUSE ANTI HUMAN CD42a:FITC; anti-CD42a antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
FMC-25
Specificity
CD42a
Form/Format
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Sequence Length
177
Applicable Applications for anti-CD42a antibody
FCM/FACS (Flow Cytometry)
Perservative Stabilisers
0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Immunogen
Peripheral blood mononuclear cells
Buffer Solution
Phosphate buffered saline
Histology Positive Control Tissue
Bone marrow
Target Species
Human
Max Ex/Em
Fluorophore: FITC ; Excitation Max (nm): 490 ; Emission Max (nm): 525
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation and Storage
Aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended
Application Data
(Human peripheral blood platelets stained with Mouse anti Human CD42a:FITC (AAA50233))
Application Data
(Human peripheral blood platelets stained with Mouse anti Human CD42a:FITC (AAA50233))
Related Product Information for anti-CD42a antibody
Mouse anti Human CD42a antibody, clone FMC-25 recognizes human CD42a, also known as Platelet glycoprotein IX, Glycoprotein 9 or GP-IX. CD42a is a 177 amino acid, ~20kDa type I single pass transmembrane glycoprotein containing a single leucine-rich repeat containing N-terminal domain and a single leucine-rich repeat containing C-terminal domain. CD42a is expressed by platelets and megakaryocytes and forms a covalent complex with CD42c (GP-1b-beta), CD42b (GP-1b-alpha) and CD42d (platelet glycoprotein V) to create the platelet surface receptor for von Willebrand factor. Incubation of the intact von Willebrand receptor complex with clone FMC-25 does not appear to inhibit binding of von Willebrand factor to the receptor (Yan et al. 2011). Defects in the GP1BB gene encoding human CD42a can lead to the inherited bleeding disorder Bernard-Soulier syndrome (Diz-Küçükkaya 2013), characterized by prolonged bleeding times, thrombocytopenia and the appearence of giant platelets in the circulation (Johns et al. 2014). Mouse anti human CD42a antibody, clone FMC-25 has been successfully used as a capture reagent for platelet-autoantibody complexes in the sera of patients presenting thrombocytopenia associated with anti-phospholipid syndrome (Godeau et al. 1997).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
platelet glycoprotein IX
NCBI Official Synonym Full Names
glycoprotein IX (platelet)
NCBI Official Symbol
GP9
NCBI Official Synonym Symbols
GPIX; CD42a
NCBI Protein Information
platelet glycoprotein IX; glycoprotein 9
UniProt Protein Name
Platelet glycoprotein IX
UniProt Gene Name
GP9
UniProt Synonym Gene Names
GP-IX; GPIX
UniProt Entry Name
GPIX_HUMAN
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Product Notes
The CD42a gp9 (Catalog #AAA50233) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD42a can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD42a gp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD42a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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