Mouse anti-Dog, Human CD42a Antibody | anti-CD42a antibody

Anti-Hu CD42a PE-Cy7

Cat. Num. AAA129349

• Gene Names: GP9; GPIX; CD42a
• Reactivity: Dog, Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Synonyms: CD42a; N/A; Anti-Hu CD42a PE-Cy7; glycoprotein IX platelet; GPIX, GP9; anti-CD42a antibody

• Host: Mouse
• Reactivity: Dog, Human
• Isotype: Mouse IgG1
• Clone Number: GR-P
• Specificity: The mouse monoclonal antibody GR-P (also known as GRP-P) recognizes an extracellular epitope of CD42a (glycoprotein 9), a 22 kDa transmembrane protein constitutively expressed on megakaryocytes and platelets.
• Purity/Purification: Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Applicable Applications for anti-CD42a antibody: FCM/FACS (Flow Cytometry)
• Immunogen: Human acute lymphoblastic leukemia cells
• Conjugation: PE-Cy7
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD45 negative CD42a positive platelets (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD42a (GR-P) PE-Cy7 antibody (4ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD42a (GR-P) PE-Cy7 antibody (4ul reagent/100ul of peripheral whole blood).)

Related Product Information for anti-CD42a antibody

CD42a, also known as glycoprotein 9 (GPIX), composes together with GPIb alpha, GPIb beta and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. Defects in the gene encoding CD42a are a cause of Bernard-Soulier syndrome, also known as giant platelet disease. These patients have unusually large platelets and have a clinical bleeding tendency.

References

*Brown SB, Clarke MC, Magowan L, Sanderson H, Savill J: Constitutive death of platelets leading to scavenger receptor-mediated phagocytosis. A caspase-independent cell clearance program. J Biol Chem. 2000 Feb 25;275(8):5987-96., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=brown+sb+clarke+mc+magowan+l ,*Vettore S, Scandellari R, Moro S, Lombardi AM, Scapin M, Randi ML, Fabris F: Novel point mutation in a leucine-rich repeat of the GPIbalpha chain of the platelet von Willebrand factor receptor, GPIb/IX/V, resulting in an inherited dominant form of Bernard-Soulier syndrome affecting two unrelated families: the N41H variant. Haematologica. 2008 Nov;93(11):1743-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/18815197 ,*Din JN, Aftab SM, Jubb AW, Carnegy FH, Lyall K, Sarma J, Newby DE, Flapan AD: Effect of moderate walnut consumption on lipid profile, arterial stiffness and platelet activation in humans. Eur J Clin Nutr. 2011 Feb;65(2):234-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=carnegy+fh+lyall+k ,*Harding SA, Din JN, Sarma J, Josephs DH, Fox KA, Newby DE: Promotion of proinflammatory interactions between platelets and monocytes by unfractionated heparin. Heart. 2006 Nov;92(11):1635-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/16709700,

NCBI and Uniprot Product Information

• NCBI GI #: 4504077
• NCBI GeneID: 2815
• NCBI Accession #: NP_000165
• NCBI GenBank Nucleotide #: NP_000165.1
• UniProt Accession #: P14770; Q14445; Q8N1D1; Q92525
• Molecular Weight: 16.8 kDa (154aa)
• NCBI Official Full Name: platelet glycoprotein IX
• NCBI Official Synonym Full Names: glycoprotein IX platelet
• NCBI Official Symbol: GP9
• NCBI Official Synonym Symbols: GPIX; CD42a
• NCBI Protein Information: platelet glycoprotein IX
• UniProt Protein Name: Platelet glycoprotein IX
• UniProt Gene Name: GP9
• UniProt Synonym Gene Names: GP-IX; GPIX