FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (2D1) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).)
Mouse anti-Human CD45 Monoclonal Antibody | anti-CD45 antibody
Anti-Hu CD45 PE
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD45, Antibody; Anti-Hu CD45 PE; protein tyrosine phosphatase receptor type C; LCA; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; LY5; B220; GP180; anti-CD45 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
2D1
Specificity
The mouse monoclonal antibody 2D1 reacts with an extracellular epitope of all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except from erythrocytes and platelets.
Purity/Purification
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Sequence Length
1306
Applicable Applications for anti-CD45 antibody
FCM/FACS (Flow Cytometry)
Immunogen
Human peripheral blood mononuclear cells
Conjugation
PE
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (2D1) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (2D1) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human CD45 positive lymphocytes (red-filled) from human CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD45 (2D1) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human CD45 positive lymphocytes (red-filled) from human CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD45 (2D1) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).)
Related Product Information for anti-CD45 antibody
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase C isoform 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase receptor type C
NCBI Official Symbol
PTPRC
NCBI Official Synonym Symbols
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Gene Name
PTPRC
UniProt Synonym Gene Names
CD45; L-CA
UniProt Entry Name
PTPRC_HUMAN
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Product Notes
The CD45 ptprc (Catalog #AAA128308) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD45 PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD45 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD45 ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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