FCM/FACS (Flow Cytometry)
(Overlay histogram showing huuman peripheral blood lymphocytes stained with CD45 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37 degree C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37 degree C. Isotype control antibody (blue line) was mouse IgG2a (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Mouse anti-Human CD45 Monoclonal Antibody | anti-CD45 antibody
Human CD45 Antibody
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
CD45, Antibody; Human CD45 Antibody; Purified Mouse Monoclonal Antibody (Mab); anti-CD45 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
4B2
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
72
Applicable Applications for anti-CD45 antibody
ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Recombinant Protein
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Overlay histogram showing huuman peripheral blood lymphocytes stained with CD45 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37 degree C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37 degree C. Isotype control antibody (blue line) was mouse IgG2a (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing huuman peripheral blood lymphocytes stained with CD45 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37 degree C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37 degree C. Isotype control antibody (blue line) was mouse IgG2a (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Product Categories/Family for anti-CD45 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
CD45, partial
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type C
NCBI Official Symbol
PTPRC
NCBI Official Synonym Symbols
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Gene Name
PTPRC
UniProt Synonym Gene Names
CD45; L-CA
Similar Products
Product Notes
The CD45 ptprc (Catalog #AAA290899) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Human CD45 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD45 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD45 ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.