FCM/FACS (Flow Cytometry)
(C57BL/6 murine splenocytes are stained with Anti-Mouse CD45R/B220 Monoclonal Antibody(Alexa Fluor 647 Conjuaged)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
Rat anti-Mouse CD45R/B220 Monoclonal Antibody | anti-CD45R/B220 antibody
Anti-Mouse CD45R/B220 Monoclonal Antibody (Alexa Fluor 647 Conjuaged)
Gene Names
Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
CD45R/B220, Antibody; Anti-Mouse CD45R/B220 Monoclonal Antibody (Alexa Fluor 647 Conjuaged); B220; CD45; GP180; LCA; T200; anti-CD45R/B220 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgM
Clone Number
RA3.3A 1/6.1
Form/Format
Liquid; PBS with 0.05% Proclin300, 1% BSA
Sequence Length
1293
Applicable Applications for anti-CD45R/B220 antibody
FCM/FACS (Flow Cytometry)
Conjugation
Alexa Fluor 647
Preparation and Storage
Store at 2-8 degree C and protected from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(C57BL/6 murine splenocytes are stained with Anti-Mouse CD45R/B220 Monoclonal Antibody(Alexa Fluor 647 Conjuaged)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
FCM/FACS (Flow Cytometry)
(C57BL/6 murine splenocytes are stained with Anti-Mouse CD45R/B220 Monoclonal Antibody(Alexa Fluor 647 Conjuaged)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
FCM/FACS (Flow Cytometry)
(Flow cytometry antibody)
FCM/FACS (Flow Cytometry)
(Flow cytometry antibody)
Related Product Information for anti-CD45R/B220 antibody
CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-2CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is eessed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.40 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources.
References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Receptor-type tyrosine-protein phosphatase C
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol
Ptprc
NCBI Official Synonym Symbols
loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Gene Name
Ptprc
UniProt Synonym Gene Names
Ly-5
UniProt Entry Name
PTPRC_MOUSE
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Product Notes
The CD45R/B220 ptprc (Catalog #AAA174638) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD45R/B220 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD45R/B220 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD45R/B220 ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45R/B220, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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