Application Data
(AlexaFluor 700 conjugated Mouse anti Human CD45RA antibody used for the phenotypic characterization of CD8 expressing T cells by flow cytometry.Image caption:CD8 T cell subpopulation distribution in the blood and endocervix.(A) A diagram of Sallusto and Lanzavecchia’s classification scheme of CD8 T cells based on CD45RA and CCR7 expression and relative perforin expression of the different subpopulation, (B) distribution of the different CD8 T cell subpopulations in a representative C. trachomatis-negative and C. trachomatis-positive women (C-D) Distribution of different CD8 T cells in the blood (C), and in the endocervix (D) of 16 women (n=16). Using flow cytometric analyses, CD8 T cells were identified in a population of cells from PBMC and endocervical cells by gating for CD3+CD8+ cells. CD8 T cells were further characterized based on CD45RA and CCR7 expression. Naïve cells are CD45RA+ CCR7+, central memory (TCM) cells are CD45RA-CCR7+, effector memory (TEM) cells are CD45RA- CCR7-, and terminally differentiated effector memory (TEMRA) cells are CD45RA+ CCR7-. The majority of CD8 T cells in the endocervix are TEM CD8 subtype.From: Ibana JA, Myers L, Porretta C, Lewis M, Taylor SN, Martin DH, Quayle AJ.The major CD8 T cell effector memory subset in the normal and Chlamydia trachomatis-infected human endocervix is low in perforin.BMC Immunol. 2012 Dec 7;13:66.)
Mouse anti-Human CD45RA Monoclonal Antibody | anti-CD45RA antibody
Mouse Anti Human CD45RA: Amethyst Orange
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use.
Application Data
(AlexaFluor 700 conjugated Mouse anti Human CD45RA antibody used for the phenotypic characterization of CD8 expressing T cells by flow cytometry.Image caption:CD8 T cell subpopulation distribution in the blood and endocervix.(A) A diagram of Sallusto and Lanzavecchia’s classification scheme of CD8 T cells based on CD45RA and CCR7 expression and relative perforin expression of the different subpopulation, (B) distribution of the different CD8 T cell subpopulations in a representative C. trachomatis-negative and C. trachomatis-positive women (C-D) Distribution of different CD8 T cells in the blood (C), and in the endocervix (D) of 16 women (n=16). Using flow cytometric analyses, CD8 T cells were identified in a population of cells from PBMC and endocervical cells by gating for CD3+CD8+ cells. CD8 T cells were further characterized based on CD45RA and CCR7 expression. Naïve cells are CD45RA+ CCR7+, central memory (TCM) cells are CD45RA-CCR7+, effector memory (TEM) cells are CD45RA- CCR7-, and terminally differentiated effector memory (TEMRA) cells are CD45RA+ CCR7-. The majority of CD8 T cells in the endocervix are TEM CD8 subtype.From: Ibana JA, Myers L, Porretta C, Lewis M, Taylor SN, Martin DH, Quayle AJ.The major CD8 T cell effector memory subset in the normal and Chlamydia trachomatis-infected human endocervix is low in perforin.BMC Immunol. 2012 Dec 7;13:66.)
Application Data
(AlexaFluor 700 conjugated Mouse anti Human CD45RA antibody used for the phenotypic characterization of CD8 expressing T cells by flow cytometry.Image caption:CD8 T cell subpopulation distribution in the blood and endocervix.(A) A diagram of Sallusto and Lanzavecchia’s classification scheme of CD8 T cells based on CD45RA and CCR7 expression and relative perforin expression of the different subpopulation, (B) distribution of the different CD8 T cell subpopulations in a representative C. trachomatis-negative and C. trachomatis-positive women (C-D) Distribution of different CD8 T cells in the blood (C), and in the endocervix (D) of 16 women (n=16). Using flow cytometric analyses, CD8 T cells were identified in a population of cells from PBMC and endocervical cells by gating for CD3+CD8+ cells. CD8 T cells were further characterized based on CD45RA and CCR7 expression. Naïve cells are CD45RA+ CCR7+, central memory (TCM) cells are CD45RA-CCR7+, effector memory (TEM) cells are CD45RA- CCR7-, and terminally differentiated effector memory (TEMRA) cells are CD45RA+ CCR7-. The majority of CD8 T cells in the endocervix are TEM CD8 subtype.From: Ibana JA, Myers L, Porretta C, Lewis M, Taylor SN, Martin DH, Quayle AJ.The major CD8 T cell effector memory subset in the normal and Chlamydia trachomatis-infected human endocervix is low in perforin.BMC Immunol. 2012 Dec 7;13:66.)
Application Data
(Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Human CD45RA)
Application Data
(Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Human CD45RA)
Application Data
(Figure A. RPE conjugated mouse anti human CD45RO and A647 conjugated mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti human CD45RO and A647 conjugated mouse anti Human CD45RA . All experiments performed on red cell lysed human blood gated on lymphocytes. Data acquired on the ZE5 cell analyzer.)
Application Data
(Figure A. RPE conjugated mouse anti human CD45RO and A647 conjugated mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti human CD45RO and A647 conjugated mouse anti Human CD45RA . All experiments performed on red cell lysed human blood gated on lymphocytes. Data acquired on the ZE5 cell analyzer.)
Application Data
(Figure A. RPE conjugated mouse anti human CD45RO and PE-A647 conjugated mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti human CD45RO and PE-A647 conjugated mouse anti Human CD45RA . All experiments performed on red cell lysed human blood gated on lymphocytes. Data acquired on the ZE5 cell analyzer.)
Application Data
(Figure A. RPE conjugated mouse anti human CD45RO and PE-A647 conjugated mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti human CD45RO and PE-A647 conjugated mouse anti Human CD45RA . All experiments performed on red cell lysed human blood gated on lymphocytes. Data acquired on the ZE5 cell analyzer.)
Application Data
(Figure A. PE-Cy5 conjugated mouse anti human CD45RO and FITC conjugated Mouse IgG1 isotype control . Figure B. PE-Cy5 conjugated mouse anti human CD45RO and FITC conjugated mouse anti human CD45RA . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock)
Application Data
(Figure A. PE-Cy5 conjugated mouse anti human CD45RO and FITC conjugated Mouse IgG1 isotype control . Figure B. PE-Cy5 conjugated mouse anti human CD45RO and FITC conjugated mouse anti human CD45RA . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock)
Application Data
(Figure A. PE conjugated Mouse anti Human CD45RO and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. PE conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human CD45RA . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Application Data
(Figure A. PE conjugated Mouse anti Human CD45RO and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. PE conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human CD45RA . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.
Mouse anti Human CD45RA antibody, clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes.
Mouse anti Human CD45RA antibody, clone F8-11-13 is routinely tested in flow cytometry on human peripheral blood lymphocytes.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The CD45RA ptprc (Catalog #AAA12272) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD45RA: Amethyst Orange reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD45RA can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Min. Dilution: Neat, Max. Dilution: 1/10 Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Researchers should empirically determine the suitability of the CD45RA ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45RA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.