Rat anti-Mouse CD49e Monoclonal Antibody | anti-CD49e antibody
Anti-Ms CD49e Purified Azide Free
Reactivity
Mouse
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Functional Assay, Immunocytochemistry
Purity
Purified by protein-A affinity chromatography
Synonyms
CD49e, Antibody; Anti-Ms CD49e Purified Azide Free; ITGA5; VLA-5 alpha; FNRA; integrin alpha 5; anti-CD49e antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a kappa
Clone Number
5H10-27 (MFR5)
Specificity
The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.
Purity/Purification
Purified by protein-A affinity chromatography
Form/Format
Purified Azide Free. Azide free phosphate buffered saline (PBS) solution, 0.2um filter sterilized
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD49e antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), FA (Functional Assay), ICC (Immunocytochemistry)
Immunogen
C57BL/6 x A/J)F1 murine mast cell line
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.
Related Product Information for anti-CD49e antibody
CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135kDa and 25kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.
References
*van der Loo JC, Xiao X, McMillin D, Hashino K, Kato I, Williams DA: VLA-5 is expressed by mouse and human long-term repopulating hematopoietic cells and mediates adhesion to extracellular matrix protein fibronectin. J Clin Invest. 1998 Sep 1;102(5):1051-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=mcmillin+,*Sampaio AL, Zahn G, Leoni G, Vossmeyer D, Christner C, Marshall JF, Perretti M: Inflammation-dependent alpha 5 beta 1 (very late antigen-5) expression on leukocytes reveals a Functional Application (FUNC)tional role for this integrin in acute peritonitis. J Leukoc Biol. 2010 May;87(5):877-84., URL: J Leukoc Biol. 2010 May;87(5):877-84.,*Plett PA, Frankovitz SM, Orschell CM: Distribution of marrow repopulating cells between bone marrow and spleen early after transplantation. Blood. 2003 Sep 15;102(6):2285-91., URL: http://www.ncbi.nlm.nih.gov/pubmed/12775569
NCBI and Uniprot Product Information
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Product Notes
The CD49e itga5 (Catalog #AAA129070) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ms CD49e Purified Azide Free reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD49e can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), FA (Functional Assay), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the CD49e itga5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD49e, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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