FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells using CD49e Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse CD49e Polyclonal Antibody | anti-ITGA5 antibody
CD49e Antibody (C-term)
Gene Names
ITGA5; FNRA; CD49e; VLA-5; VLA5A
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CD49e, Antibody; CD49e Antibody (C-term); Integrin alpha-5; CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD49e; Integrin alpha-5 heavy chain; Integrin alpha-5 light chain; ITGA5; FNRA; anti-ITGA5 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CD49e antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 796-824 amino acids from the C-terminal region of human CD49e.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
796-824
Sequence Length
1,049
Applicable Applications for anti-ITGA5 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells using CD49e Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells using CD49e Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of CD49e antibody (C-term) in mouse bladder tissue lysates (35ug/lane). CD49e (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of CD49e antibody (C-term) in mouse bladder tissue lysates (35ug/lane). CD49e (arrow) was detected using the purified Pab.)
Related Product Information for anti-ITGA5 antibody
CD49e belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein is the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Product Categories/Family for anti-ITGA5 antibody
References
Boehmler,A.M., J. Immunol. 182 (11), 6789-6798 (2009)
Okazaki,T., Am. J. Pathol. 174 (6), 2378-2387 (2009)
Schornberg,K.L., Proc. Natl. Acad. Sci. U.S.A. 106 (19), 8003-8008 (2009)
Okazaki,T., Am. J. Pathol. 174 (6), 2378-2387 (2009)
Schornberg,K.L., Proc. Natl. Acad. Sci. U.S.A. 106 (19), 8003-8008 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
114536
NCBI Official Full Name
integrin alpha-5
NCBI Official Synonym Full Names
integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
NCBI Official Symbol
ITGA5
NCBI Official Synonym Symbols
FNRA; CD49e; VLA-5; VLA5A
NCBI Protein Information
integrin alpha-5
UniProt Protein Name
Integrin alpha-5
UniProt Gene Name
ITGA5
UniProt Synonym Gene Names
FNRA
UniProt Entry Name
ITA5_HUMAN
Similar Products
Product Notes
The ITGA5 itga5 (Catalog #AAA283877) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 796-824. The CD49e Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD49e can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ITGA5 itga5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD49e, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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