Application Data
(Staining of frozen rat spleen with Mouse anti Rat CD54)
Mouse CD54 Monoclonal Antibody | anti-CD54 antibody
MOUSE ANTI RAT CD54
Gene Names
Icam1; CD54; ICAM
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
CD54, Antibody; MOUSE ANTI RAT CD54; anti-CD54 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1A29
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
545
Applicable Applications for anti-CD54 antibody
WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Preparation
Immunogen
Rat Ax cells (a HEV derived cell line)
Fusion Partners
Fusion Partners
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Staining of frozen rat spleen with Mouse anti Rat CD54)
Application Data
(Staining of frozen rat spleen with Mouse anti Rat CD54)
Application Data
(Staining of stimulated rat spleen cells with Mouse anti Rat CD54:FITC)
Application Data
(Staining of stimulated rat spleen cells with Mouse anti Rat CD54:FITC)
Application Data
(Staining of stimulated rat spleen cells with Mouse anti Rat CD54:Alexa Fluor 488 (AAA49442A488))
Application Data
(Staining of stimulated rat spleen cells with Mouse anti Rat CD54:Alexa Fluor 488 (AAA49442A488))
Related Product Information for anti-CD54 antibody
Mouse anti Rat CD54 antibody, clone 1A29 recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a 90kD adhesion molecule belonging to the immunoglobulin superfamily. CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system. Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling (Etienne, S. et al.). Functionally 1A29 inhibits homotypic aggregation of PHA blasts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60,142 Da
NCBI Official Full Name
intercellular adhesion molecule 1
NCBI Official Synonym Full Names
intercellular adhesion molecule 1
NCBI Official Symbol
Icam1
NCBI Official Synonym Symbols
CD54; ICAM
NCBI Protein Information
intercellular adhesion molecule 1; ICAM-1
UniProt Protein Name
Intercellular adhesion molecule 1
UniProt Gene Name
Icam1
UniProt Synonym Gene Names
Icam-1; ICAM-1
UniProt Entry Name
ICAM1_RAT
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Product Notes
The CD54 icam1 (Catalog #AAA49442) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD54 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD54 icam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD54, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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