Application Data
(Published customer image: ICAM-1 activates PKCa but not PKCbetaII in HMVECLs. A) At 70% confluence, HMVECLs cells pretreated with 10 ng/ml TNFa to induce ICAM-1 expression. At 24 hrs, the cells were suspended and immunolabeled with anti-ICAM-1 antibodies and examined by flow cytometry for ICAM-1 expression. B -D) HMVECLs were pretreated with TNFa as in panel A. At 24 hrs, the endothelial cells were nonstimulated (NS) or stimulated with a confluent monolayer of anti-ICAM-1-coated beads or the control anti-PECAM-1-coated beads for 20 minutes in B and D or for the times indicated in C. The cells were lysed and the activation of PKCbetaII and PKCa was examined by western blot with B) anti-phospho-PKCbetaIIThr641 or anti-PKCbetaII or C -D) anti-phospho- PKCaThr638 or anti-PKCa. Shown are representative blots. Shown are the mean +/- SEM of 3 experiments. NT, nontreated. *, p)
Mouse anti-Dog CD54 Monoclonal Antibody | anti-CD54 antibody
MOUSE ANTI HUMAN CD54
Gene Names
ICAM1; BB2; CD54; P3.58
Reactivity
Dog
Applications
Immunoprecipitation, Functional Assay, Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry
Synonyms
CD54, Antibody; MOUSE ANTI HUMAN CD54; anti-CD54 antibody
Host
Mouse
Reactivity
Dog
Clonality
Monoclonal
Isotype
IgG1
Clone Number
84H10
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
532
Applicable Applications for anti-CD54 antibody
IP (Immunoprecipitation), FA (Functional Assay), FCM/FACS (Flow Cytometry), ELISA, IHC (Immunohistochemistry)
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Immunogen
K562 cell line
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the MOPC 315 mouse myeloma cell line.
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Data
(Published customer image: ICAM-1 activates PKCa but not PKCbetaII in HMVECLs. A) At 70% confluence, HMVECLs cells pretreated with 10 ng/ml TNFa to induce ICAM-1 expression. At 24 hrs, the cells were suspended and immunolabeled with anti-ICAM-1 antibodies and examined by flow cytometry for ICAM-1 expression. B -D) HMVECLs were pretreated with TNFa as in panel A. At 24 hrs, the endothelial cells were nonstimulated (NS) or stimulated with a confluent monolayer of anti-ICAM-1-coated beads or the control anti-PECAM-1-coated beads for 20 minutes in B and D or for the times indicated in C. The cells were lysed and the activation of PKCbetaII and PKCa was examined by western blot with B) anti-phospho-PKCbetaIIThr641 or anti-PKCbetaII or C -D) anti-phospho- PKCaThr638 or anti-PKCa. Shown are representative blots. Shown are the mean +/- SEM of 3 experiments. NT, nontreated. *, p)
Application Data
(Published customer image: ICAM-1 activates PKCa but not PKCbetaII in HMVECLs. A) At 70% confluence, HMVECLs cells pretreated with 10 ng/ml TNFa to induce ICAM-1 expression. At 24 hrs, the cells were suspended and immunolabeled with anti-ICAM-1 antibodies and examined by flow cytometry for ICAM-1 expression. B -D) HMVECLs were pretreated with TNFa as in panel A. At 24 hrs, the endothelial cells were nonstimulated (NS) or stimulated with a confluent monolayer of anti-ICAM-1-coated beads or the control anti-PECAM-1-coated beads for 20 minutes in B and D or for the times indicated in C. The cells were lysed and the activation of PKCbetaII and PKCa was examined by western blot with B) anti-phospho-PKCbetaIIThr641 or anti-PKCbetaII or C -D) anti-phospho- PKCaThr638 or anti-PKCa. Shown are representative blots. Shown are the mean +/- SEM of 3 experiments. NT, nontreated. *, p)
Related Product Information for anti-CD54 antibody
Mouse anti Human CD54 antibody, clone 84H10 recognizes the D1 domain of ICAM-1.
It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Mouse anti Human CD54 antibody, clone 84H10 detects an antigen of ~90 kDa.
Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1.
Mouse anti Human CD54 antibody, clone 84H10 is routinely tested in flow cytometry on rat splenocytes.
It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Mouse anti Human CD54 antibody, clone 84H10 detects an antigen of ~90 kDa.
Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1.
Mouse anti Human CD54 antibody, clone 84H10 is routinely tested in flow cytometry on rat splenocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
intercellular adhesion molecule 1
NCBI Official Synonym Full Names
intercellular adhesion molecule 1
NCBI Official Symbol
ICAM1
NCBI Official Synonym Symbols
BB2; CD54; P3.58
NCBI Protein Information
intercellular adhesion molecule 1; ICAM-1; cell surface glycoprotein P3.58; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor
UniProt Protein Name
Intercellular adhesion molecule 1
UniProt Gene Name
ICAM1
UniProt Synonym Gene Names
ICAM-1
UniProt Entry Name
ICAM1_HUMAN
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Product Notes
The CD54 icam1 (Catalog #AAA49422) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CD54 reacts with Dog and may cross-react with other species as described in the data sheet. AAA Biotech's CD54 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FA (Functional Assay), FCM/FACS (Flow Cytometry), ELISA, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD54 icam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD54, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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